Project description:Mapping of transposon mutant library during growth in Brain Heart Infusion (BHI) broth and in a rat endocarditis model. The goal of this study was to identify factors that play a role in E. faecium endocarditis by selection of transposon insertion mutants that lost the capacity to cause infections.
Project description:Q fever is due to Coxiella burnetii, an obligate intracellular bacterium. We investigated the mechanism of establishment of chronic form of the Q fever that mainlym anifested by endocarditis. We showed that patients with acute Q fever and valvulopathy, who have the higher risk to develop an endocarditis, exhibited high levels of circulating apoptotic cells. We further investigated the effect of the uptake of dead cells on the intracellular fate of the bacterium and the immune response of monocytes and macrophages.
Project description:Transcriptional profiles of cardiac valves from patients with bacterial infectious endocarditis (3 infected with Streptococcus sp. and 2 infected with Staphylococcus aureus) were compared with uninfected cardiac valves (7 patients) from patients with hemodynamic disturbing events
