Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing analysis of bladder tissue from CFT073-infected C57B/6J mice with or without treatments of D-xyl@epsilonPLCDs and epsilonPLCDs

ABSTRACT: RNA sequencing analysis of bladder tissue from CFT073-infected C57B/6J mice with or without treatments of D-xyl@epsilonPLCDs and epsilonPLCDs

PROVIDER: PRJNA991491 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25145028_1.fastq.gz	Fastqsanger.gz
	SRR25145028_2.fastq.gz	Fastqsanger.gz
	SRR25145029_1.fastq.gz	Fastqsanger.gz
	SRR25145029_2.fastq.gz	Fastqsanger.gz
	SRR25145030_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

RNA sequencing analysis of bladder tissue from CFT073-infected C57B/6J mice with or without treatments of D-xyl@epsilonPLCDs and epsilonPLCDs

Project description:We designed D-xylose-modified, epsilon-poly-L-lysine-based carbon dots (D-xyl@epsilonPLCDs) to effectively inhibit intracellular UPEC. Treatment with D-xyl@epsilonPLCDs ultimately resulted in a reduction of UPEC in the bladders of mouse UTIs model. To investigate the regulation of immune responses in bladders of CFT073-infected mice post-D-xyl@epsilonPLCDs treatment, RNA sequencing analysis were performed.

2024-07-23 | GSE236506 | GEO

Genome wide binding sites of NsrR in uropathogenic E.coli CFT073

2015-06-15 | GSE69829 | GEO

The role of NLRP3 in regulation of antimicrobial peptides and estrogen signaling in UPEC-infected bladder epithelial cells

Project description:The NLRP3 inflammasome, estrogen and antimicrobial peptides have all been emphasised to have a vital role in the protection of the bladder urothelium. However, the interdependence between these protective factors during a bladder infection is currently unknown. Our aim was to investigate the role of NLRP3 in regulation of antimicrobial peptides and estrogen signaling in bladder epithelial cells during a UPEC infection. Human bladder epithelial cells and CRISPR/Cas9 generated NLRP3-deficient cells were stimulated with the UPEC strain CFT073 and estradiol. The gene and protein expression were evaluated with microarray, qRT-PCR, western blot and ELISA. Microarray results showed that the expression of most antimicrobial peptides was reduced in CFT073-infected NLRP3-deficient cells compared to Cas9 control cells. Conditioned medium from NLRP3-deficient cells also lost the ability to suppress CFT073 growth. Moreover, NLRP3-deficient cells had lower basal release of Beta-defensin-1, Beta-defensin-2 and RNase7. The ability of estradiol to induce an increased expression of antimicrobial peptides was also abrogated in NLRP3-deficient cells. The decreased antimicrobial peptide expression might be linked to the observed reduced expression and activity of estradiol receptor beta in NLRP3-deficient cells. This study suggests that NLRP3 may regulate the release and expression of antimicrobial peptides and affect estrogen signaling in bladder epithelial cells.

2023-10-04 | GSE243098 | GEO

RNAseq-based bladder cancer subtyping and IPA analysis with the xenografts from C57 BL/6J

Project description:To subtype the Triple gene knockout tumor we generated and assess the similiarities with widely used MB49 cells, TKO cells were injected into bladder wall orthtopically and bladder lumen by catheter intravesically in C57 BL/6J, while MB49 intravesical injection as a control. TKO intravesical tumors, TKO orthotopic tumors and MB49 intravesical tumors were collected and sent for RNA-seq analysis. In results, both intravesical TKO and orthotopic TKO tumors demonstrates transcriptome profiles that closely resembled human basal like muscle invasive bladder cancer using a luminal/basal/neuronal classifier. Principal Component Analysis (PCA) across samples demonstrated that MB49 cells had transcriptome profiles that differed significantly from TKO tumors. Hence, this study suggest that the TKO tumors closely resemble basal like human bladder cancer and MB49 exhibited no expression of urothelial markers.

2022-06-01 | GSE200005 | GEO

Escherichia coli CFT073

Project description:Genome wide binding sites of NsrR in uropathogenic E.coli CFT073

| PRJNA286884 | ENA

Genome wide binding sites of NsrR in uropathogenic E.coli CFT073

Project description:We applied ChIP-seq to identify genome wide binding targets of NsrR in E.coli CFT073. NsrR is a nitric oxide sensitive regulator of transcription. Genome wide binding targets of NsrR have been identified in E.coli K12 using ChIP-chip. The genome of CFT073 is about 0.6Mb larger than that of K12. In this study, we identify the novel NsrR binding sites in CFT073. The nsrR gene was modified by the addition of DNA sequences encoding a C-terminal 3X-Flag tag, and the tagged gene was integrated into the chromosome. NsrR bound DNA was isolated by chromatin immunoprecipitation and it was sequenced using Miseq platform.

2015-06-15 | E-GEOD-69829 | biostudies-arrayexpress

Escherichia coli CFT073 isolate:199310

Project description:Escherichia coli CFT073 isolate:199310 Genome sequencing

| PRJNA624646 | ENA

Escherichia coli CFT073 isolate:PU_38

Project description:Escherichia coli CFT073 isolate:PU_38 Genome sequencing

| PRJNA387644 | ENA

miRNA profiles of lung tissues from Mtb H37Rv infected C57B/6 mice and uninfected controls

Project description:The C57B/6 mice were infected with Mtb H37Rv (CFU=200) for 28 days, and the miRNA expression profile from lung tissues were analyzed with deep sequencing.

2018-09-06 | GSE119496 | GEO

Mus musculus strain:C57B/L

Project description:Mus musculus strain:C57B/L Raw sequence reads

| PRJNA299277 | ENA