Municipal solid waste and leachate
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA991727 | ENA |
REPOSITORIES: ENA
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Project description:solid waste metagenome Raw sequence reads
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Project description:We reported the gene expression profile of T47D cells treated with the organic extract of Particulate matter 2.5 (PM2.5) sampled next to the municipal solid waste incineration plant of Bologna city. Based on a air pollution distribution model that takes the incinaration plant as point source of emission, two sites were chosen to sample particulate matter near incineration plant: "FrulloEst" representing the maximum effect of the incineration plant, "Calamosco" representing the negative control of "FrulloEst" (minimun effect of incineration plant, same effect of other air pollution fonts). Another site, "Giardini Margherita", is chosen to sample the urban background air pollution. for each site sample collection was performed in winter and in summer season.
2018-01-31 | E-MEXP-3686 | biostudies-arrayexpress
Project description:solid waste treatment microorganism Raw sequence reads
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Project description:This work aimed at studying the photochemical treatment of a landfill leachate using ultraviolet light, hydrogen peroxide, and ferrous or ferric ions, in a batch recycle photoreactor. The effect of inorganic carbon presence, pH, initial H2O2 amount (0-9990 mg L-1) as well as Fe(II) (200-600 ppm) and Fe(III) (300-700 ppm) concentrations on the total carbon removal and color change was studied. Prior to the photochemical treatment, a pretreatment process was applied; inorganic nitrogen and inorganic carbon were removed by means of air stripping and initial pH regulation, respectively. The leachate sent subsequently for photochemical treatment was free of inorganic carbon and contained only organic carbon with concentration 1200±100 mg L-1 at pH 5.1-5.3. The most favorable concentrations of H2O2 and ferric ions for carbon removal were 6660 mg L-1 and 400 ppm, respectively. Adjusting the initial pH value in the range of 2.2-5.3 had a significant effect on the organic carbon removal. The photo-Fenton-like process was more advantageous than the photo-Fenton one for leachate treatment. By applying the most favorable operating conditions, 88.7% removal of total organic carbon, 100% removal of total inorganic carbon, 96.5% removal of total nitrogen, and 98.2% color removal were achieved.
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Project description:North Eastern United States municipal landfill leachate metagenomic assembly
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Project description:Microbial community structure and diversity in a solid waste landfill
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Project description:Landfill leachate water is often treated in a biological processing step. In most cases a stable operation of the industrial scale plants is controlled by sum parameters such as process relevant ion concentrations, dry matter concentration and dissolved oxygen concentration. A deeper understanding of the current status of the individual cell or the biocoenosis would help to understand malfunctions or the reason for inefficient plant performance. In a simple batch experimental setup, samples of two different conditions have been generated to unravel bacterial proteome changes in response to medium term lack of oxygen supply and landfill leachate addition. The first condition was an activated sludge sample condition from an industrial scale landfill leachate treatment plant with the process stages of nitrification and denitrification. After 45 days without aeration and with addition of leachate and carbon sources as fed batch, the second sample (condition 2) was taken. A comprehensive LC-MS/MS based protemic screen was performed aiming for the identification and quantification of waste water specific bacteria proteomes. To this end, a novel combination of two protein extraction methods has been established meeting the requirements for LC-MS/MS anaylsis. Around 600 proteins were identified of which 90 % were quantified in at least 3 replicates. Numerous essential proteins to maintain the cell redox homeostasis are overexpressed in the condition 1 which was aerated with oxygen and stressed by the ultrafiltration compared to condition 2, which was not aerated in a lab experiment. In addition, heat and cold shock proteins and two proteins related to the apoptosis of organisms (spermidine/putrescine transport system and apoptosis-inducing factor) were identified.
2022-02-23 | PXD011917 | Pride