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Modification of liver transcriptome under the influence of ascites triggered by cold ambient temperature

ABSTRACT: Modification of liver transcriptome under the influence of ascites triggered by cold ambient temperature

PROVIDER: PRJNA992563 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25204933_1.fastq.gz	Fastqsanger.gz
	SRR25204933_2.fastq.gz	Fastqsanger.gz
	SRR25204934_1.fastq.gz	Fastqsanger.gz
	SRR25204934_2.fastq.gz	Fastqsanger.gz
	SRR25204935_1.fastq.gz	Fastqsanger.gz

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Modification of liver transcriptome under the influence of ascites triggered by cold ambient temperature

Project description:Ascites syndrome in both human and chicken has been traditionally known as a metabolic disorder. However, recent scientific findings have suggested a genetic base for the susceptibility to develop AS. The complexity of molecular mechanisms underlying the influence of AS on chicken main organs has not been comprehensively elucidated. In this work, after precise differentiating the ascitic chickens from those healthy ones, we conducted a transcriptome profile comparison between the ascitic and healthy chickens within both males and females using high-throughput RNA-seq data. The results revealed a total of 1,152 differentially expressed genes (DEGs; 669 up- and 483 down-regulated) in males and 1,803 DEGs in females (489 up- and 1,314 down-regulated) between the ascitic and healthy chickens. There were 450 DEGs in common in both males and females.

2023-08-01 | GSE236885 | GEO

Ascites-derived circulating microRNAs as potential diagnostic biomarkers of gastric cancer-associated malignant ascites

Project description:Peritoneal carcinomatosis with malignant ascites is associated with dismal prognosis in gastric cancer. Malignant ascites is the most relevant body fluid in which to seek diagnostic biomarkers for peritoneal carcinomatosis. We aimed to identify and validate ascites-derived circulating microRNAs (miRNAs) that are differentially expressed between liver cirrhosis-associated benign ascites (LC-ascites) and gastric cancer-associated malignant ascites (GC-ascites). MiRNA expression levels were investigated in three independent cohorts. Overall, 165 ascites samples (73 LC-ascites and 92 GC-ascites) were obtained from the National Biobank of Korea. Initially, microarrays were used to screen the expression levels of 2,006 miRNAs in the discovery cohort (n = 22). Subsequently, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analyses were used to validate the expression levels of selected miRNAs in the training (n = 70) and validation (n= 73) cohorts. In addition, the levels of carcinoembryonic antigen (CEA), a commonly used tumor marker, were determined in the ascites samples. Expression levels of miR-574-3p, miR-181b-5p, miR-4481, and miR-181d were significantly lower in the GC-ascites samples than in the LC-ascites samples, and miR-181b-5p showed the best diagnostic performance for GC-ascites (area under the curve [AUC] = 0.798 and 0.846 for the training and validation cohorts, respectively). The diagnostic performance of CEA for GC-ascites was improved if CEA and miR-181b-5p were analyzed together (AUC = 0.981 and 0.946 for the training and validation cohorts, respectively). Overall, we identified ascites-derived circulating miRNAs capable of differentiating non-malignant ascites and GC-ascites, and demonstrated that the combined use of miR-181b-5p and CEA produces the optimal diagnostic yield.

2019-02-12 | GSE126399 | GEO

Long-Term Storage of Human Cells at Ambient Temperature

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H3K36me3 histone modification and regulation of ambient temperature-induced alternative splicing in Arabidopsis

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Expression data of ascites treated peritoneal carcinomatosis human cell lines

Project description:Characterizations of ascites proteome from ovarian PC and gastric PC have demonstrated that ascites contains elevated pro-tumorigenic factors. Reasoning that the composition of ascitic fluid might offer insight into the memory of key biological events occurring intra-abdominally, we hypothesized that paracrine factors essential for survival and growth of peritoneal deposits are secreted into and circulate within ascitic fluid. Our data from cytokine array profile suggest that ascites contains biologically active ligands capable of supporting cellular functions of cancer cells. To decipher downstream signalling pathways activated in cancer cells when exposed to ascites, we performed gene expression analysis of Colo-205 and SNU-C1 cells upon exposure to PC ascites.

2022-02-22 | GSE189004 | GEO

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