Project description:Renewal of the catecholamine-secreting chromaffin cell population of the adrenal medulla is necessary for physiological homeostasis throughout life. Definitive evidence for the presence or absence of an adrenomedullary stem cell has been enigmatic. In this work, we show that a subpopulation of uncommitted sustentacular cells, previously thought to be support cells, are in fact adrenomedullary stem cells. These postnatal cells express the transcription factor SOX2, and give rise to chromaffin cells of both the adrenaline and noradrenaline lineages in vivo and in vitro. We present comprehensive transcriptomic data of the mouse adrenal medulla as well as of purified SOX2+ adrenomedullary stem cell populations. Through genetic tracing, we show that postnatal stem cells specialise from embryonic Schwann Cell Precursors and identify this population in the normal adult human adrenal medulla.
Project description:Transcriptom analysis of microdissect adrenal medulla after 8 weeks of cardiac pressure overload caused by transverse aortic constriction. Comparative transcriptome analysis was determined using the GeneChip Mouse Genome 430 2.0 Array (Affymetrix, Santa Clara, CA, USA). Six microarrays from microdissected adrenal medulla of mice were performed 8 weeks after transverse aortic constriction or sham operation.
Project description:Transcriptom analysis of microdissect adrenal medulla after 8 weeks of cardiac pressure overload caused by transverse aortic constriction.
Project description:Multipotent stem cells are considered as promising biological tools for regenerative medicine, and transcriptome analysis of these cells is fundamental for identifying new factors controlling proliferation versus differentiation. To obtain a comprehensive characterization of adrenal progenitors, we have developed an analytical pipeline relying on flow cytometric sorting of living cells followed by RNA sequencing. This analysis showed that two distinct populations of Nestin-positive progenitors are located in the adrenal cortex and medulla, respectively. Though 1039 genes were differentially regulated, both populations showed Schwann cell precursor properties suggesting both populations to be neural crest-derived.
Project description:The carotid body is a chemoreceptor that senses decreases in blood oxygen to increase breathing in hypoxia. To look for candidate oxygen sensors in the carotid body, we compared the gene expression of the carotid body to the adrenal medulla, a similar tissue that does not have oxygen sensitivity in adults. For each sample, we pooled 18 carotid bodies or 10 adrenal medullas from 10 adult mice. Group numbers designate the same cohort of animals.
Project description:The carotid body is a chemoreceptor that senses decreases in blood oxygen to increase breathing in hypoxia. To look for candidate oxygen sensors in the carotid body, we compared the gene expression of the carotid body to the adrenal medulla, a similar tissue that does not have oxygen sensitivity in adults. For each sample, we pooled 18 carotid bodies and 10 adrenal medullas from 10 adult mice.
Project description:Microarray profiling single and repeated immobilization stress responses in gene expression in rat adrenal medulla Experiment Overall Design: Immobilization stress (IMO) was performed with 2-hour once and 2-hour daily for 6 days repeated. Control groups were not exposed to stress (absolute controls). Following the last IMO, rats were euthanized by decapitation and both adrenal medulla tissues were dissected from the animal. Total RNA were isolated and the array experiments were done by Affymetrix. Data analysis will be performed by GeneTraffic program.
Project description:Genome-wide expression profiling showed significant overlap of the genetic networks regulated by butyrate in vivo (rat adrenal medulla) and in vitro (PC12 cells) including TH and other neurotransmitter-related genes (DBH, AADC, GTPCH, ppEnk, NPY). Experiment Overall Design: Studies in PC12 cells were performed to delineate which genetic networks are regulated by SCFA depending on the dose. Experiments in vivo were performed to test wheter similar phenomenon operates in the sympathoadrenal system. Experiment Overall Design: ISBPC: 1mMSB PC12 Experiment Overall Design: GSBPC: 6mMSB PC12 Experiment Overall Design: CPCR: CPC12 Experiment Overall Design: SBAM: rat SBAdrenal Medulla Experiment Overall Design: CAM: rat Control Adrenal Medulla
Project description:The aim of this study is to establish a comprehensive transcriptome atlas that enables identification of key molecular pathways and morphogenic events regulating postnatal renal medulla/papillary and cortex development. To achieve this, a microarray expression profiling was performed on postnatal day 0-90 renal medulla and cortex obtained from CD1 male mice.