Project description:Tumor-derived cell lines are used as in vitro cancer models, but their ability to accurately reflect the phenotype and genotype of the parental histology remains questionable, given the prevalence of documented cell line–specific cytogenetic changes. We have addressed the issue of whether copy number alterations seen in tumor-derived cell lines reflect those observed in studies of fresh tissue by carrying out a meta-analysis of array-based comparative genomic hybridization data that considers both copy number alteration frequencies and the occurrence of cancer gene amplifications and homozygous deletions. Keywords: comparative genomic hybridization
Project description:Array-based comparative genomic hybridization (array CGH) analyses was used to analyze 17 VSCC samples from untreated patients. The data were validated using quantitative PCR.
Project description:To directly compare genomic alterations between patient tumors and derived cell lines, both parental tumors and cell lines were characterized by array-based comparative genomic hybridization.
Project description:Array-based comparative genomic hybridization (array CGH) analyses was used to analyze 20 UPS and 17 LMS samples from untreated patients. The data were validated using quantitative PCR.
Project description:Characterization of copy number alterations and unbalanced breakpoints in human esophageal squamous cell carcinoma cell lines by array-based comparative genomic hybridization.
Project description:In this study, we investigated amplifications and deletions of 49 gastric cancer cell lines by 244k oligonucleotide-based array comparative genomic hybridization.