Project description:Purpose: RNA-Seq was used to detect the dwonstream genes after FASN knockdow. Method: Three chondrocyte total RNA profiles of Si-NC samples and Si-FASN samples were analyzed.
Project description:Purpose: RNA-Seq was used to detect the dwonstream genes after CircRREB1 knockdow. Method: Three chondrocyte total RNA profiles of Si-NC samples and Si-CircRREB1 samples were analyzed.
Project description:To investigate the mRNA profiling in RKO and SW620 cells with or without shRNA-mediated METTL17 knockdown, we performed RNA-seq with the total RNA extracted from the CRC cells.
Project description:Smad4 is a central mediator of canonical TGF/BMP signaling and plays important roles in mesenchymal cell aggregation, chondrocyte differentiation, osteoblast differentiation and maturation. However, the regulatory mechanism of Smad4 underlying chondrocyte hypertrophy during skeletal development is unknown. To elucidate the molecular mechanism by which Smad4 deficiency impairs chondrocyte hypertrophy, we performed high-throughput RNA-seq to identify target genes involved in Smad4-regulated chondrocyte hypertrophy. Our results suggest that Smad4 controls chondrocyte hypertrophy through regulating Runx2 expression during skeletal development.