Project description:Oleocanthal (OL) and Oleacein (OC) are enriched components in Olive (oil and leaves). However, their effects on skin have not been widely studied compared to Oleuropein (OP). Here, we perfomed global gene expression profiling to screen the potential effects of OL and OC on the skin. Results showed that OL and OC have effects on skin development and keratinocyte differentiation by upregulation of key markers. Furthermore, OL and OC were effectively downregulated several melanogenic genes and it suggests that OL and OC can have a potential effect for pigmentation. B16F10 were murine melanoma cells line, treated with 5 µM of OL and OC for 24 hours. Microarray gene expression profiling was conducted technical replicates.
Project description:Gene expression profiling reveals a potential role of plumbagin on the induction of B16F10 metastasis cells were treated with 0 and 2 µM plumbagin for 24 h ; Microarray gene expression profiling was conducted.
Project description:Gene expression profiling reveals a potential role of Orange cells (BT-OC) in stimulating hair growth in dermal papilla cells. HFDPCs were human primary cells line, treated with 1:2000 Orange cells (BT-OC) for 48 h. Microarray gene expression profiling was conducted for three biological replicates
Project description:Gene expression profiling reveals a potential role of Butyroside D in melanogenesis inhibition in B16F10 cells. B16F10 cells were murine melanoma cell line, treated with 2 μM Butyroside D for 48 h. Microarray gene expression profiling was conducted for two biological replicates
Project description:Gene expression profiling reveals a potential role of Butyroside D in melanogenesis inhibition in B16F10 cells. B16F10 cells were murine melanoma cell line, treated with 0.2 μM Butyroside D for 48 h. Microarray gene expression profiling was conducted for two biological replicates
Project description:Global gene expression profiling reveals a potential anti-melanoma effect of SQ-diEG in B16F10 cells. Squalene (SQ) was considered as a promising natural agent in anti-cancer treatment due to its strong antioxidant and anti-inflammatory activity; however, its pharmacological value has been largely underestimated because of its poor solubility and bioavailability. To adress this problem, a novel amphiphilic SQ derivative which bearing ethylene glycol oligomers was synthesized and was used as a permeation enhancer in this study to check its potential effect on anti-melanoma using B16F10 cells B16F10 were murine melanoma cells line, treated with 2.5 µM and 40 µM with all samples for 48 h. Microarray gene expression profiling was conducted for two biological replicates