Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Direct reprogramming of fibroblasts into functional macrophages

ABSTRACT: Direct reprogramming of fibroblasts into functional macrophages

PROVIDER: PRJNA996042 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Direct reprogramming of fibroblasts into functional macrophages [RNA-Seq]

Project description:Direct reprogramming of fibroblasts into functional macrophages [RNA-Seq]

| PRJNA996047 | ENA

Direct reprogramming of fibroblasts into functional macrophages [scRNA-Seq]

Project description:Direct reprogramming of fibroblasts into functional macrophages [scRNA-Seq]

| PRJNA996045 | ENA

Direct reprogramming of fibroblasts into functional macrophages [RNA-Seq]

Project description:Macrophages derived from somatic cells by reprogramming technologies have the potential to enable the development of cell-based therapies for numerous malignant diseases. Here we report on the establishment of a novel methodology allowing for the conversion of fibroblasts into functional macrophages with pro-inflammatory phenotype just by c-Myc overexpression. Ectopic expression of c-Myc in fibroblasts in induced pluripotent stem cell (iPSC) culture medium induced the rapid appearance of CD45+ hematopoietic cell complex (HCC) intermediates with engraftment capacity as well as the repopulation of distant hematopoietic compartments. HCC intermediates were stably maintained in suspension culture and continuously generated functional and highly pure-induced macrophage (iMac) just by M-CSF cytokine stimulation. Single-cell transcriptomic analysis of HCC intermediates revealed that c-Myc up-regulates the expression of MafB, a major regulator of macrophage differentiation, to promote macrophage differentiation. Characterization of the macrophage activity showed NF-kB signaling activation and pro-inflammatory phenotype. iMac transplantation significantly reduced leukemia and breast cancer progression in animal xenograft models. Our finding indicates that reprogramming strategies of fibroblasts could help circumvent long-standing obstacles to gaining “off-the-shelf” macrophages for anti-cancer immunotherapy.

2024-09-18 | GSE237620 | GEO

Direct reprogramming of fibroblasts into functional macrophages [scRNA-Seq]

2024-09-18 | GSE237619 | GEO

Direct reprogramming of mouse fibroblasts into functional skeletal muscle progenitors

Project description:Direct reprogramming of mouse fibroblasts into functional skeletal muscle progenitors

| PRJNA427588 | ENA

Ultra-Efficient Exosome-Guided Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes

Project description:Ultra-Efficient Exosome-Guided Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes

| PRJNA699851 | ENA

Direct Reprogramming of Fibroblasts into Functional Mammary Epithelial cells by small molecules

Project description:Direct Reprogramming of Fibroblasts into Functional Mammary Epithelial cells by small molecules

| PRJNA597354 | ENA

Direct Reprogramming of Fibroblasts into Functional Mammary Epithelial cells by small molecules (WGBSseq)

Project description:Direct Reprogramming of Fibroblasts into Functional Mammary Epithelial cells by small molecules (WGBSseq)

| PRJNA597357 | ENA

Direct Reprogramming of Fibroblasts into Functional Mammary Epithelial cells by small molecules (lncRNAseq)

Project description:Direct Reprogramming of Fibroblasts into Functional Mammary Epithelial cells by small molecules (lncRNAseq)

| PRJNA597360 | ENA

Ultra-Efficient Exosome-Guided Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes [miRNA-Seq]

Project description:Ultra-Efficient Exosome-Guided Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes [miRNA-Seq]

| PRJNA699853 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data