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Absence of chromosome axis proteins recruitment prevents meiotic recombination chromosome-wide in the budding yeast Lachancea kluyveri (CC-Seq)

ABSTRACT: Absence of chromosome axis proteins recruitment prevents meiotic recombination chromosome-wide in the budding yeast Lachancea kluyveri (CC-Seq)

PROVIDER: PRJNA996192 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25324893_1.fastq.gz	Fastqsanger.gz
	SRR25324893_2.fastq.gz	Fastqsanger.gz
	SRR25324894_1.fastq.gz	Fastqsanger.gz
	SRR25324894_2.fastq.gz	Fastqsanger.gz

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Absence of chromosome axis proteins recruitment prevents meiotic recombination chromosome-wide in the budding yeast Lachancea kluyveri (Hi-C)

Project description:Absence of chromosome axis proteins recruitment prevents meiotic recombination chromosome-wide in the budding yeast Lachancea kluyveri

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Absence of chromosome axis proteins recruitment prevents meiotic recombination chromosome-wide in the budding yeast Lachancea kluyveri (CC-Seq)

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Absence of chromosome axis proteins recruitment prevents meiotic recombination chromosome-wide in the budding yeast Lachancea kluyveri (ChIP-Seq)

Project description:Here we studied the budding yeast Lachancea kluyveri, a cousin of the model Saccharomyces cerevisiae, in order to try to understand the mechanism responsible for the absence of meiotic recombination on its almost entire sex chromosome (Brion et al. 2017). We performed protein-DNA interaction mapping of Rec8, Red1, Hop1 and Zip3 proteins by ChIP-seq. Briefly, we observed an enrichment of Zip3, a protein known as a marker of type I crossing-overs, where peaks of DSBs were detected by CC-seq and a virtual absence of Zip3 within Lakl0C-left. For Rec8, a meiotic specific protein part of the cohesin complex, we detected enriched peaks at centromeres and inside intergenic regions flanked by convergent ORFs everywhere in the genome Lakl0C-left included. Remarkably, Red1 and Hop1, known to form the lateral element of the synaptonemal complex and important for DSB formation, were enriched mainly at Rec8 peaks except at centromeres and particurlarly on Lakl0C-left where they are completely absent.

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Iron-responsive genes in Lachancea kluyveri

Project description:To understand the iron-responsive gene expression in Lachancea kluyveri under high and low iron conditions, we perfomred the genome-wide gene expression profiling for the log-phase cells of Lachancea kluyveri wild type, sef1 deletion, aft1 deletion and sef1aft1 double deletion mutants under the YPD+400mM ferrous iron and YPD+ 200 mM BPS conditions.

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Sef1-dependent gene expression in Lachancea kluyveri

Project description:To understand the Sef1-dependent gene expression in Lachancea kluyveri under both fermentative and respiratory conditions, we perfomred the genome-wide gene expression profiling for the log-phase cells of Lachancea kluyveri wild type and sef1 deletion mutant under both YPD and YPGly conditions.

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Sef1-VP16 dependent gene expression in Lachancea kluyveri

Project description:To understand the highly active Sef1-VP16 dependent gene expression in Lachancea kluyveri under both fermentative and respiratory conditions, we perfomred the genome-wide gene expression profiling for the log-phase cells of Lachancea kluyveri wild type and SEF1-VP16 gain-of-function mutant under both YPD and YPGly conditions.

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Absence of chromosome axis proteins recruitment prevents meiotic recombination chromosome-wide in the budding yeast Lachancea kluyveri

Project description:Absence of chromosome axis proteins recruitment prevents meiotic recombination chromosome-wide in the budding yeast Lachancea kluyveri

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Lachancea kluyveri

Project description:Petite-negative budding yeast (known as Lachancea kluyveri or Saccharomyces kluyveri) sequenced for comparative genomics studies

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Project description:Transcriptional profiling of the yeast Lachancea kluyveri when grown on minimal media with different compounds added as the sole nitrogen source. Cells grown on uracil, uridine, dihydrouracil and ammonia were profiled using cells grown on proline as the reference.

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Absence of chromosome axis proteins recruitment prevents meiotic recombination chromosome-wide in the budding yeast Lachancea kluyveri (ChIP-Seq)

Project description:Absence of chromosome axis proteins recruitment prevents meiotic recombination chromosome-wide in the budding yeast Lachancea kluyveri (ChIP-Seq)

| PRJNA996191 | ENA

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