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ScRNAseq of Hematopoietic stem cell populations from human cord blood

ABSTRACT: scRNAseq of Hematopoietic stem cell populations from human cord blood

PROVIDER: PRJNA996900 | ENA |

REPOSITORIES: ENA

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scRNAseq of Hematopoietic stem cell populations from human cord blood

Project description:Single cell transcriptomic profiling (sc RNA-seq) of the two Human Hematopoietic Stem Cell Populations from cord blood: 18LinnegCD34posCD133pos (hereafter CBC1:CD34pos ) and 18LinnegCD34negCD133pos HSCs (hereafter CBC3:CD34neg). Purpose: to compare the single cells transcriptomic profiles of the two Human Hematopoietic Stem Cell Populations: CD34pos HSCs and CD34neg HSCs in order to find unique homing molecules profile. Results provide insight of highly expressed adhesion molecules in CD34neg HSCs and their crucial role in interacting with the bone marrow microenvironment.

2024-02-14 | GSE237832 | GEO

RNA-Seq of sorted human cord-blood derived hematopoietic cell populations

Project description:RNA-Seq of sorted human cord-blood derived hematopoietic cell populations

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Gene Expression Study of Background, Progenitor and Stem Cell Populations from Human Umbilical Cord Blood

Project description:Gene expression study of populations of primary human hematopoietic cells from human cord blood including background, progenitor and stem cells. Keywords: other

2008-02-13 | GSE10438 | GEO

scRNAseq of innate lymphoid cells generated in vitro from umbilical cord blood derived CD34+ hematopoietic progenitors.

Project description:Innate lymphoid cells (ILCs) were generated in vitro from CD34+ hematopoietic progenitors derived from umbilical cord blood, and RNA sequencing was performed on CD45+ LineageLD- cells to assess transcriptional identities of lineages generated

2021-08-25 | GSE171114 | GEO

Transcriptomic analysis of human hematopoietic populations sorted from umbilical cord blood.

Project description:We isolated by fluorescence-activated cell sorting highly purified populations (long term hematopoietic stem cells (LT-HSCs), short term hematopoietic stem cells (ST-HSCs), multipotent progenitors (MPPs), common myeloid progenitor (CMPs), granulocyte and monocyte progenitors (GMPs), multilymphoid progenitors (MLPs), Myeloid-erythorid Progenitor (MEP), Granulocytes, Monocytes, B cells, T cells, Dendritic cells, Natural Killer cells and Erythrocyte Progenitors from 3 to 4 cord blood pools. We extracted RNA from 5K cells of each population and performed RNA-sequencing.

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scRNAseq of innate lymphoid cells generated in vitro from umbilical cord blood derived CD34+ hematopoietic progenitors.

Project description:scRNAseq of innate lymphoid cells generated in vitro from umbilical cord blood derived CD34+ hematopoietic progenitors.

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Impact of red blood cells on genome-wide DNA methylation and expression analyses of cord blood hematopoietic cells

Project description:Genome-wide DNA methylation (DNAm) studies have been extremely useful to understand hematopoiesis in humans. For cell-specific epigenetic studies, fluorescence-activated cell sorting (FACS) is the gold standard technique to isolate homogeneous cell populations of interest. However, in cord blood, the DNAm signature of isolated hematopoietic cells may be significantly altered by heterotopic interactions with nucleated red blood cells (nRBCs) that go undetected during conventional cell sorting. Using the Illumina 450K array, genome-wide DNAm profiles of the following cell types were obtained: (1) T lymphocytes, monocytes, and nRBCs isolated using a “standard” strategy lacking exclusion for erythroid lineage markers; and (2) CD4 and CD8 T lymphocytes, B lymphocytes, Natural Killer (NK) cells, granulocytes, monocytes, and nRBCs isolated using a “stringent” strategy formally excluding erythroid lineage-specific markers. DNAm profiles of cord blood cells isolated by the standard sorting strategy showed significant heterotopic cross-contamination between cell populations, whereas the cord blood cells sorted by the stringent strategy displayed DNAm profiles more consistent with their expected hematopoietic lineage relationships.

2015-10-02 | GSE68456 | GEO

Transcription profiling of human umbilical cord blood TO study expression OF background, progenitor AND stem cell populations

Project description:Gene expression study of populations of primary human hematopoietic cells from human cord blood including background, progenitor and stem cells. Experiment Overall Design: This experiment include 4 samples each with 3 replicates on 2 platforms for a total of 24 GEO Samples

2008-04-09 | E-GEOD-10438 | biostudies-arrayexpress

Expression data of Mesenchymal Stroma Cells (MSC)-adherent and non-adherent, human cord blood-derived, hematopoietic CD34 cells

Project description:MSC-adherent hematopoietic stem and progenotir cells (HSPC) express adhesion-associated genes at higher levels than non-adherent cells while cell-cycle and differentiation-associated genes are not significantly changed between the two cell populations. We used microarray to confirm identity of MSC-adherent and non-adherent cord blood-derived HSPCs and to exclude that cell cycle and differentiation affect adhesive capacity. CD34 positive cells were isolated from human cord blood (not older than 24h), expanded for 3 days and assayed for the adhesion to MSC. The adherent and non-adherent live CD34+ cells were sorted and total RNA was extracted.

2016-05-16 | E-GEOD-78216 | biostudies-arrayexpress

RNA-Seq of sorted human cord-blood derived hematopoietic cell populations

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