Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chrysomya megacephala

ABSTRACT: Microbe sample from intestinal microorganisms of Chrysomya megacephala Raw sequence reads

PROVIDER: PRJNA997617 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25997300_1.fastq.gz	Fastqsanger.gz
	SRR25997300_2.fastq.gz	Fastqsanger.gz
	SRR25997301_1.fastq.gz	Fastqsanger.gz
	SRR25997301_2.fastq.gz	Fastqsanger.gz
	SRR25997302_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 208

Similar Datasets

Chrysomya megacephala

Project description:Chrysomya megacephala overview

| PRJNA182804 | ENA

Chrysomya megacephala

Project description:Chrysomya megacephala Transcriptomes

| PRJNA267753 | ENA

Chrysomya megacephala

Project description:Chrysomya megacephala Genome sequencing and assembly

| PRJNA1121482 | ENA

Chrysomya megacephala

Project description:Chrysomya megacephala Transcriptome or Gene expression

| PRJNA182208 | ENA

Wolbachia endosymbiont of Chrysomya megacephala

Project description:Wolbachia endosymbiont of Chrysomya megacephala

| PRJNA385248 | ENA

Chrysomya villeneuvi

Project description:Chrysomya villeneuvi Raw sequence reads

| PRJNA700714 | ENA

Chrysomya pinguis

Project description:Chrysomya pinguis Raw sequence reads

| PRJNA777290 | ENA

Subacronicta megacephala

Project description:Subacronicta megacephala overview

| PRJNA995872 | ENA

Reference gene stability of a synanthropic fly, Chrysomya megacephala.

Project description:Stable reference genes are essential for accurate normalization in gene expression studies with reverse transcription quantitative polymerase chain reaction (qPCR). A synanthropic fly, Chrysomya megacephala, is a well known medical vector and forensic indicator. Unfortunately, previous studies did not look at the stability of reference genes used in C. megacephala.In this study, the expression level of Actin, ribosomal protein L8 (Rpl8), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 1? (EF1), ?-tubulin (?-TUB), ?-tubulin (?-TUB), TATA binding box (TBP), 18S rRNA (18S) and ribosomal protein S7 (Rps7) were evaluated for their stability using online software RefFinder, which combines the normal software of the ?Ct method, BestKeeper, Normfinder, and geNorm. Moreover the number of suitable reference gene pairs was also suggested by Excel-based geNorm. The expression levels of these reference genes were evaluated under different experimental conditions with special perspectives of forensic applications: developmental stages (eggs, first, second and third instar larvae, pupae and adults); food sources of larvae (pork, fish and chicken); feeding larvae with drugs (untreated control, Estazolam and Marvelon); feeding larvae with heavy metals (untreated control, cadmium and zinc); tissues of adults (head, thorax, abdomen, legs and wings). According to RefFinder, EF1 was the most suitable reference gene of developmental stages, food and tissues; 18S and GAPDH were the most suitable reference genes for drugs and heavy metals, respectively, which could be widely used for quantification of target gene expression with qPCR in C. megacephala. Suitable reference gene pairs were also suggested by geNorm.This fundamental but vital work should facilitate the gene studies of related biological processes and deepen the understanding in physiology, toxicology, and especially medical and forensic entomology of C. megacephala.

| S-EPMC4625446 | biostudies-literature

Chrysomya saffranea

Project description:Chrysomya saffranea overview

| PRJNA182859 | ENA