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β-glucan alleviates infective intestinal inflammation via activating Th17 cells in a teleost fish

ABSTRACT: β-glucan alleviates infective intestinal inflammation via activating Th17 cells in a teleost fish

PROVIDER: PRJNA997908 | ENA |

REPOSITORIES: ENA

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β-glucan alleviates infective intestinal inflammation via activating Th17 cells in a teleost fish

Project description:We established a bacteria infective intestinal inflammation in turbot (Scophthalmus maximus). And found that β-glucan could significantly alleviate the phenotype of turbot intestinal inflammation. We performed single cell transcriptome analysis to study bacteria infective intestinal inflammation and the effects of β-glucan. Furthermore, we revealed that β-glucan through activates Th17 cells to alleviate intestinal inflammation in turbot.

2023-12-31 | GSE238068 | GEO

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Project description:We establish a trained immunity activation model in turbot (Scophthalmus maximus) by training with β-glucan in vivo. Through single cell RNA-sequencing analysis, we annotate 16 clusters of immune cells and blood cells from head kidney and spleen, and successfully characterize that neutrophils exhibit distinguished feature of trained immunity.

2022-06-01 | GSE195628 | GEO

Single-Cell Transcriptomic Analysis Reveals Neutrophil as Orchestrator during β-Glucan-Induced Trained Immunity in a Teleost Fish

Project description:Single-Cell Transcriptomic Analysis Reveals Neutrophil as Orchestrator during β-Glucan-Induced Trained Immunity in a Teleost Fish

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Genome-wide analysis of mDC transcriptional response to cyclic glucan

Project description:Analysis of transcriptional profiles in mDC sorted from apheresed PBMC and stimulated for 6 hours with cyclic glucan or LPS. The hypothesis tested is that cyclic glucan induces maturation and T cell-activation transcripts in human mDC. Total RNA extracted from mDC sorted from apheresed PBMC and activated for 6 hours with cyclic glucan or LPS.

2012-12-31 | E-GEOD-32023 | biostudies-arrayexpress

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The role of HIF-1 in beta-glucan induced response in myeloid cell

Project description:beta-glucan induced glycolysis in HIF-1 depedent manner. We reported that beta-glucan injection in mice led to upregulated glycolysis. HIF-1a plays a major role in this process. Mice receives beta-glucan via ip for 4 days. Splenocytes were isolated for RNA sequencing.

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Project description:A comparative epigenomic and single-cell transcriptomic analysis of teleost regeneration

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RNASeq of bone marrow-derived macrophages treated with beta-glucan and lipopolysaccharide (LPS)

Project description:We found that beta-glucan treatment induces early induction of transcripts associated with inflammation and metabolism. Several days after beta-glucan treatment, genes associated with differentation and housekeeping remain upregulated. LPS treatment induces a strong inflammatory response. Beta-glucan did not significantly alter the macrophage response to LPS.

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