Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing (RNA-seq) of ANKRD6 in MKN45 gastric cancer cells by shRNA

ABSTRACT: RNA sequencing (RNA-seq) of ANKRD6 in MKN45 gastric cancer cells by shRNA

PROVIDER: PRJNA997969 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25436800_1.fastq.gz	Fastqsanger.gz
	SRR25436800_2.fastq.gz	Fastqsanger.gz
	SRR25436801_1.fastq.gz	Fastqsanger.gz
	SRR25436801_2.fastq.gz	Fastqsanger.gz
	SRR25436802_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Human Gastric Cancer Cell line MKN45: Non-Target shRNA vs. SET/I2PP2A targeting shRNA

Project description:Transcriptional profiling of MKN45 cells comparing control stably expressing Non-Targeting shRNA cells with stably expressing SET/I2PP2A targeting shRNA cells. Goal was to determine the effects of SET knockdown on MKN45 gene expression.

2016-09-06 | GSE86437 | GEO

RNA transcriptome sequencing analysis of MKN45 cells transfected with tcons_00001221 shRNA or control shRNA

Project description:An RNA transcriptome sequencing analysis was performed in MKN45 cells that were transfected with tcons_00001221 shRNA or control shRNA.

2018-05-12 | GSE114360 | GEO

RNA transcriptome sequencing analysis of MKN45 cells transfected with tcons_00001221 shRNA or control shRNA

Project description:RNA transcriptome sequencing analysis of MKN45 cells transfected with tcons_00001221 shRNA or control shRNA

| PRJNA471072 | ENA

Human gastric cancer cell MKN45: MKN45-GFP control vs. highly metastatic sublines

2011-11-10 | GSE33570 | GEO

Human gastric cancer cell MKN45: MKN45-GFP control vs. highly metastatic sublines

Project description:Gene expression profiles of in vitro selected highly metastatic MKN45-GFP sublines. The results were compared with MKN45-GFP control cell line to determine the metastasis associated genes. Four pairs compared experiment. Each pair was used MKN45-GFP cells as correlated control. Determining on the gene expression trends were by various metastatic ability of each subline.

2011-11-10 | E-GEOD-33570 | biostudies-arrayexpress

RNA sequencing (RNA-seq) of ANKRD6 in MKN45 gastric cancer cells by shRNA

Project description:Metastasis and recurrence are the major causes of poor outcome in gastric cancer (GC). We showed that ANKRD6 expression is associated with a poorer prognosis in 10 independent GC cohorts and with metastatic states and processes. Here, by transcriptomic profiling we have characterized the effect of ANKRD6 silencing in vitro and found that cells with ANKRD6 downregulation display an impairment in key metastatic processes such as the Epithelial-mesenchymal transition.

2024-04-10 | GSE238103 | GEO

Anti-proliferation activity of fucoidan on MKN45 gastric cancer cells and downregulation of phosphorylated ASK1, a cell-cycle-regulated cyclin-dependent kinase

Project description:Fucoidan is a high-molecular polysaccharide whose main constituent is sulfated fucose. Extensive studies have demonstrated numerous interesting biological activities for fucoidan. We specifically focused on the anti-proliferation activity of fucoidan and examined the underlying mechanism in MKN45 gastric cancer cells. BrdU assay revealed that fucoidan impeded the MKN45 cell cycle by approximately 50%, and clonogenic assay also showed that fucoidan inhibited cell proliferation. Preliminary examinations of fucoidan using LDH assay showed no immediate cytotoxic effects at 24-h exposure. However, longer time courses revealed inhibition of cell growth at 4 days in a dose-dependent manner. Microarray analysis in MKN45 cells treated with fucoidan identified genes that were upregulated and downregulated in response to fucoidan, including MAP3K5, or ASK1 (apoptosis signal-regulating kinase), which was upregulated by 1.38-fold. Western blot confirmed that fucoidan increased ASK1 protein levels, while reducing the levels of phosphorylated ASK1. Reduction of ASK1 by siRNA decreased proliferation of MKN45 cells. Our findings show that fucoidan may suppress cellular proliferation and DNA synthesis in MKN45 cells by suppressing the ASK1-p38 signaling pathway through reduction of phosphorylated ASK1 levels.

2017-02-09 | GSE58774 | GEO

Expression data from human stomach cell lines (MKN45 and MKN45P)

Project description:Analysis to find splicing variants that are differentially expressed in a highly metastatic stomach cancer cell line, MKN45P, versus its parental cell line, MKN45 Comparison between highly metastatic gastric cancer cell line MKN45P and its parental cell line MKN45

2010-06-08 | E-GEOD-21572 | biostudies-arrayexpress

Genes regulated by SET7/9 knockdown in gastric cancer cells

Project description:Expression of SET7/9, a histone methyltransferase, was frequently reduced in cultured and primary gastric cancers. To identify the SET7/9 target genes in gastric cancer, we performed siRNA-based knockdown of SET7/9 in MKN74 and MKN45 cells and then examined significant expression changes of genes by microarray. Transfection of SET7/9 siRNA into MKN74 and MKN45 cells were performed by electroporation. After 48hrs, cells were harvested. Total RNA was used for cDNA microarray.

2015-07-24 | E-GEOD-59834 | biostudies-arrayexpress

mRNA sequence Analysis of Apocynin treated MKN45

Project description:Noxo1, a component of NADPH oxidase 1 (NOX1) complex, is upregulated in gastric cancer cells in a inflammation-dependent manner, and plays an important role in tumorigenesis (Oncogene, 33: 3820, 2014). To examine the mechanism of NOX1/ROS signaling in tumorigenesis, MKN45 gastric cancer cells were treated with apocynin, an inhibitor for NOX, and their gene expression was examined by RNA sequencing. Based on expression data, Sox2 was shown to be suppressed by apocynin, suggesting a role of Sox2 in a inflammation-associated gastric tumorigenesis.

2019-01-27 | GSE111520 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data