Project description:Dmrt1 (doublesex and mab-3 related transcription factor 1) is a conserved transcriptional regulator of male differentiation required for testicular development in vertebrates. This study examines the result of conditional removal of Dmrt1 from Sertoli cells in P28 testis tissue. Testes from P28 Dmrt1 flox/flox mice were compared to testes from P28 Dmrt1 flox/flox mice with the Sertoli cell-specific Sf1 (Nr5a1; MGI:1346833) or Dhh (MGI:94891) promoters driving Cre expression.
Project description:In order to identify cell-autonomous factors that might be regulating the neurogenic niche, type 1 and 2 progenitors were FACS sorted from P7 and P28 animals and their cDNA was hybridized to a stem cell-specific microarray. Keywords: differential gene expression analysis Two samples per microarray (P7 and P28). Each microarray experiment was done in triplicate. P7 is the control sample.
Project description:In order to identify cell-autonomous factors that might be regulating the neurogenic niche, type 1 and 2 progenitors were FACS sorted from P7 and P28 animals and their cDNA was hybridized to a stem cell-specific microarray. Keywords: differential gene expression analysis
Project description:Dmrt1 (doublesex and mab-3 related transcription factor 1) is a conserved transcriptional regulator of male differentiation required for testicular development in vertebrates. This study examines the result of conditional removal of Dmrt1 from Sertoli cells in P28 testis tissue.
