Arabidopsis thaliana
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: E-GEOD-7641E-GEOD-10496
PROVIDER: PRJNA99837 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Expression analysis of root cell types after treatment with low pH
| PRJNA155173 | ENA
Project description:Expression analysis of root cell-types after iron deficiency (-Fe) treatment
| PRJNA108933 | ENA
Project description:Expression analysis of root cell types after treatment with sulfur deficient media
| PRJNA155181 | ENA
Project description:Expression analysis of root pericycle cell-types after iron deficiency (-Fe) treatment
| PRJNA129515 | ENA
Project description:Expression analysis of root developmental zones after treatment with salt
| PRJNA99773 | ENA
Project description:Microarray data were generated to investigate the changes in gene expression for two cell types of the root: pericycle and cortical cells in response to salt treatment. The transcription profile of both cell types were also generated. Cell-types of interest expressing specifically Green Fluorescent Protein (GFP) were isolated from the rest of the root using fluorescence-activated cell sorting (FACS).
2014-04-10 | GSE44307 | GEO
Project description:Microarray data were generated to investigate the changes in gene expression for two cell types of the root: pericycle and cortical cells in response to salt treatment. The transcription profile of both cell types were also generated. Cell-types of interest expressing specifically Green Fluorescent Protein (GFP) were isolated from the rest of the root using fluorescence-activated cell sorting (FACS). 11 arrays were generated from plants grown in control and salt conditions. All seedlings grew in one tank were collected and correspond to one biological sample. Two control biological replicates were used for pericycle and 3 for cortical cells. By comparison from control microarray, 6 others arrays from salt treated plant (3 for each cell types) were used to identified salt responsive genes. The salt treatment was done on day 6 and 7 with increment of 25mM every 24h.
2014-04-10 | E-GEOD-44307 | biostudies-arrayexpress
Project description:We performed an expression analysis of the response of seedling root tips to 1 hour of treatment with 140mM NaCl using mutants defective in root hair patterning. Cells are amazingly adept at integrating both external and internal cues to regulate transcriptional states. While internal processes such as differentiation and cell-type specification are generally understood to have an important impact on gene expression, very little is known about how cells utilize these developmental cues to regulate responses to external stimuli. Here we use the response to a well characterized environmental stress, high salinity, to obtain a global view of the role that cell identity plays in guiding transcriptional responses in the root of Arabidopsis. Our analysis is based on three microarray data sets we have generated that explore transcriptional changes spatially among 6 cell layers and 4 longitudinal regions or temporally along 5 time points after salt treatment. We show that the majority of the response to salt stress is cell-type specific resulting in the differential regulation of unique biological functions in subsets of cell layers. To understand the regulatory mechanisms controlling these responses we have analyzed cis-element enrichment in the promoters of salt responsive genes and demonstrate that known stress regulatory elements likely control responses to salt occurring in multiple cell types. Despite the extensive shift in transcriptional state that salt stress elicits, we are able to identify several biological processes that consistently define each cell layer and find that transcriptional regulators of cell-identity tend to exhibit robust cell-type specific expression. Finally, using mutants that disrupt cell-type specification in the epidermis, we reveal cell autonomous and non-autonomous effects when cell identity is altered. Together, these data elucidate a novel intersection between physiology and development and expand our understanding of how transcriptional states are regulated in a multi-cellular context. Keywords: Response analysis of mutants
2008-04-24 | GSE8787 | GEO
Project description:Expression analysis of root developmental zones after iron deficiency (-Fe) treatment
| PRJNA108929 | ENA
Project description:Salt treatment of PtSnRK2.7 overexpressor
| PRJNA317590 | ENA