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H3K27me3 ChIP-seq on 16 days after pollination endosperm of cultivated barley

ABSTRACT: H3K27me3 ChIP-seq on 16 days after pollination endosperm of cultivated barley

PROVIDER: PRJNA998412 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25423337_1.fastq.gz	Fastqsanger.gz
	SRR25423337_2.fastq.gz	Fastqsanger.gz
	SRR25423338_1.fastq.gz	Fastqsanger.gz
	SRR25423338_2.fastq.gz	Fastqsanger.gz
	SRR25423339_1.fastq.gz	Fastqsanger.gz

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Project description:The nuclear content of the plant endosperm is the result of the contribution two maternal genomes and a single paternal genome. This 2:1 dosage relationship provides a unique system for studying the additivity of gene expression levels in reciprocal hybrids. A combination of microarray profiling and allele-specific expression analysis was performed using RNA isolated from endosperm tissues of maize inbred lines B73 and Mo17 and their reciprocal hybrids at two developmental stages, 13 and 19 days after pollination. By assessing the relative levels of expression in the reciprocal hybrids it was possible to determine the prevalence of additive and non-additive expression patterns. While the majority of differentially expressed genes displayed additive expression patterns in the endosperm, approximately 10% of the genes displayed non-additive expression patterns including maternal-like, paternal-like, dominant high-parent, dominant low-parent and expression patterns outside the range of the inbreds. The frequency of hybrid expression patterns outside of the parental range in maize endosperm tissue is much higher than that observed for vegetative tissues. For a set of 90 genes allele-specific expression assays were employed to monitor allelic bias and regulatory variation. Eight of these genes exhibited evidence for maternally or paternally biased expression at multiple stages of endosperm development and are potential examples of differential imprinting. Collectively, our data indicate that parental effects on gene expression are much stronger in endosperm than in vegetative tissues, and that endosperm imprinting may be far more common than previously estimated. Experiment Overall Design: Affymetrix microarrays were used to perform expression profiling on 13 day after pollination endosperm tissue of four different genotypes; B73; Mo17, B73xMo17 and Mo17xB73. There are three biological replicates for each of the tissues. Each biological sample represents a pool containing 5 endosperms each, from 6 different ears.

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H3K27me3 ChIP-seq on 16 days after pollination endosperm of cultivated barley

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