Project description:Genome wide DNA methylation profiling of glioma patient surgical samples and corresponding xenotransplants and cell lines. The Illumina Infinium 450k Human DNA methylation Beadchip and the Illumina EPIC Beadchip were used to obtain DNA methylation profiles. Samples include 22 patient surgical samples, 26 xenografted patient tumors, and 6 cell lines derived there-of.
Project description:Primary outcome(s): The incidence of surgical site infections after laparoscopic colorectal surgery using monofilament absorbable suture material within 30 days after surgery
Project description:Interventions: oral antibiotics
Primary outcome(s): The rate of surgical site infection at 30th days after surgery
Study Design: Single arm Non-randomized
Project description:4 Adult male Sprague-Dawley rats (275-350 g) were anesthetized and subjected to hepatectomy sham surgery (abdominal cavity was opened, liver was handled, but no tissue resection was made). 1 hour after the surgery rats were killed and liver samples were harvested. This study was conducted to analyzes the effects of surgical stress on gene expression levels in rat liver. It provides additional data to 1-6 h partial hepatectomy study (Series GSE7415). Keywords: 1h hepatectomy sham surgery
Project description:Interventions: oral antibiotics
Primary outcome(s): The rate of surgical site infection at 30th days after surgery
Study Design: Single arm Non-randomized
Project description:Study designQuestionnaire-based survey.ObjectivesSurgical site infection (SSI) is a common complication in spine surgery but universal guidelines for SSI prevention are lacking. The objectives of this study are to depict a global status quo on implemented prevention strategies in spine surgery, common themes of practice and determine key areas for future research.MethodsAn 80-item survey was distributed among spine surgeons worldwide via email. The questionnaire was designed and approved by an International Consensus Group on spine SSI. Consensus was defined as more than 60% of participants agreeing to a specific prevention strategy.ResultsFour hundred seventy-two surgeons participated in the survey. Screening for Staphylococcus aureus (SA) is not common, whereas preoperative decolonization is performed in almost half of all hospitals. Body mass index (BMI) was not important for surgery planning. In contrast, elevated HbA1c level and hypoalbuminemia were often considered as reasons to postpone surgery. Cefazoline is the common drug for antimicrobial prophylaxis. Alcohol-based chlorhexidine is mainly used for skin disinfection. Double-gloving, wound irrigation, and tissue-conserving surgical techniques are routine in the operating room (OR). Local antibiotic administration is not common. Wound closure techniques and postoperative wound dressing routines vary greatly between the participating institutions.ConclusionsWith this study we provide an international overview on the heterogeneity of SSI prevention strategies in spine surgery. We demonstrated a large heterogeneity for pre-, peri- and postoperative measures to prevent SSI. Our data illustrated the need for developing universal guidelines and for testing areas of controversy in prospective clinical trials.
Project description:Liver tissue from three-spine stickleback individuals from 3 populations were tested on arrays to determine array suitability for transcriptomics experiments.