Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation

ABSTRACT: Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation

PROVIDER: PRJNA998703 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation [scRNA-seq]

Project description:Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation [scRNA-seq]

| PRJNA998714 | ENA

Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation [Multiome-seq]

Project description:Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation [Multiome-seq]

| PRJNA998715 | ENA

Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation [Cut&Run]

Project description:Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation [Cut&Run]

| PRJNA998713 | ENA

Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation [ChIP-seq]

Project description:Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation [ChIP-seq]

| PRJNA998712 | ENA

Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation [bulk RNA-seq]

Project description:Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation [bulk RNA-seq]

| PRJNA998711 | ENA

Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation [bulk ATAC-seq]

Project description:Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation [bulk ATAC-seq]

| PRJNA998710 | ENA

Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation

Project description:Enteroendocrine cells (EECs) secrete serotonin (enterochromaffin [EC] cells) or specific peptide hormones (non-EC cells) that serve vital metabolic functions. The basis for terminal EEC diversity remains obscure. By forcing activity of the transcription factor (TF) NEUROG3 in 2D cultures of human intestinal stem cells, we replicated physiologic EEC differentiation and examined transcriptional and cis-regulatory dynamics that culminate in discrete cell types. Abundant EEC precursors expressed stage-specific genes and TFs. Before expressing pre-terminal NEUROD1, post-mitotic precursors oscillated between transcriptionally distinct ASCL1+ and HES6hi cell states. Loss of either factor accelerated EEC differentiation substantially and disrupted EEC individuality; ASCL1 or NEUROD1 deficiency had opposing consequences on EC and non-EC cell features. These TFs mainly bind cis-elements that are accessible in undifferentiated stem cells, and they tailor subsequent expression of TF combinations that underlie discrete EEC identities. Thus, early TF oscillations retard EEC maturation to enable accurate diversity within a medically important cell lineage.

2024-01-10 | GSE238276 | GEO

Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation [Cut&Run]

Project description:To investigate the regulation of human enteroendocrine cell differentiation, we established Neurog3ER inducible cell lines. We then analyzed ASCL1 and NEUROD1 binding using data obtained from CUT&RUN of at 96h.

2024-01-11 | GSE238272 | GEO

Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation [ChIP-seq]

Project description:To investigate the regulation of human enteroendocrine cell differentiation, we established Neurog3ER inducible cell lines. We then analysed active histone marks using data obtained from H3K27ac ChIP-seq at various time points.

2024-01-11 | GSE238271 | GEO

Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation [Multiome-seq]

Project description:To investigate the regulation of human enteroendocrine cell differentiation, we established Neurog3ER inducible cell lines. We then performed simultaneous gene expression profiling and chromatin analysis at single cell level using data obtained from Multiome-seq at various time points.

2024-01-11 | GSE238275 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data