Project description:Pseudomonas extremaustralis an Antarctic bacterium was grown at low oxygen conditions and exposed to oxidative stress during 1 hour. Experimental and control samples were analyzed by RNA-seq experiments.

2020-03-30 | E-MTAB-7712 | biostudies-arrayexpress

RNA-seq of Pseudomonas extremaustralis/pBAD18-sRNA40 and P. extremaustralis/pBAD18 strain

Project description:sRNA40 (a novel sRNA) was pulse-expressed and its effects studied by transcriptomic profiling in P. extremaustralis, an Antarctic bacterium. For this approach we built a plasmid-based system, where sRNA40 was cloned under the control of the heterologous araBp promoter, inducible by arabinose (pBAD18-sRNA40). P. extremaustralis carrying the empty plasmid pBAD (pBAD18) was used as a control. Both strains were grown at low oxygen conditions for 24 hours. Cells were harvested after 10 min of induction with 0.1% arabinose. RNA was isolated using the Trizol method. RNA quality was analyzed on the Agilent Bioanalyzer and rRNA depletion was performed using the RiboZERO (Illumina). Samples were validated using an Agilent 2100 Bioanalyzer (Agilent Technologies). Libraries were prepared with TruSeq RNA Library Prep Kit v2 (Illumina) and sequenced with the Illumina NextSeq 550 platform with a single-end protocol.

2023-04-29 | E-MTAB-10406 | biostudies-arrayexpress

Pseudomonas extremaustralis strain:DSM 17835 | isolate:DSM 17835

Project description:Pseudomonas extremaustralis DSM 17835 genome sequencing

| PRJNA319695 | ENA

RNA-seq of Pseudomonas extremaustralis/pBAD18-sRNA40 and P. extremaustralis/pBAD18 strain

Project description:RNA-seq of Pseudomonas extremaustralis/pBAD18-sRNA40 and P. extremaustralis/pBAD18 strain

| PRJEB44663 | ENA

Pseudomonas extremaustralis strain:USBA 515 | isolate:CMPUJ U 515

Project description:Pseudomonas extremaustralis USBA 515 genome sequencing

| PRJNA330486 | ENA

Pseudomonas extremaustralis strain:2E-UNGS | isolate:Rio Reconquista

Project description:Pseudomonas extremaustralis strain:2E-UNGS | isolate:Rio Reconquista Genome sequencing