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Janus nanoparticles targeting extracellular polymeric substance achieve flexible elimination of drug-resistant biofilms

ABSTRACT: Janus nanoparticles targeting extracellular polymeric substance achieve flexible elimination of drug-resistant biofilms

PROVIDER: PRJNA999229 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25437729_1.fastq.gz	Fastqsanger.gz
	SRR25437729_2.fastq.gz	Fastqsanger.gz
	SRR25437730_1.fastq.gz	Fastqsanger.gz
	SRR25437730_2.fastq.gz	Fastqsanger.gz
	SRR25437731_1.fastq.gz	Fastqsanger.gz

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Janus nanoparticles targeting extracellular polymeric substance achieve flexible elimination of drug-resistant biofilms

Project description:Safe and efficient antibacterial materials are urgently needed to combat drug-resistant bacteria and biofilm-associated infections. The rational design of nanoparticles for flexible elimination of biofilms by alternative strategies remains challenging. Herein, we propose the fabrication of Janus-structured nanoparticles targeting extracellular polymeric substance to achieve dispersion or near-infrared (NIR) light-activated photothermal elimination of drug-resistant biofilms, respectively. Asymmetrical Janus-structured dextran-bismuth selenide (Dex-BSe) nanoparticles are fabricated by a facile strategy to exploit synergistic effects of both components. The biocompatible dextran domain with the maximum exposure endows the Janus nanoparticles with biofilm penetration, targeting, and dispersion functions. Interestingly, Janus Dex-BSe nanoparticles realize enhanced dispersal of biofilms over time compared with dextran nanoparticles while the underlying molecular mechanisms are further revealed by RNA-sequencing transcriptomics analysis. Alternatively, taking advantage of the preferential accumulation of nanoparticles at infection sites, the self-propelled active motion induced by the unique Janus structure enhances the photothermal killing effect under NIR light irradiation, thereby eradicating the biofilm. Given these favorable features, the antibiofilm activity of Janus Dex-BSe against methicillin-resistant Staphylococcus aureus (MRSA) was first validated in vitro. More importantly, the flexible application of Janus Dex-BSe nanoparticles for biofilm removal or NIR-triggered eradication in vivo was demonstrated by MRSA-infected mouse excisional wound model and abscess model, respectively. The currently developed Janus nanoplatform holds great promise for the efficient elimination of drug-resistant biofilms in diverse antibacterial scenarios.

2023-08-01 | GSE239411 | GEO

Janus nanoparticles targeting extracellular polymeric substance achieve flexible elimination of drug-resistant biofilms.

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JANUS, a spliceosome-associated protein, promotes miRNA biogenesis in Arabidopsis

Project description:MicroRNAs (miRNAs) are important regulators of gene expression. Their levels are precisely controlled through modulating the activity of the microprocesser complex (MC). Here we report that JANUS, a homology of the conserved U2 snRNP assembly factor in yeast and human, is required for miRNA accumulation. JANUS associates with MC components Dicer-like 1 and SERRATE (SE) and directly binds the stem-loop of pri-miRNAs. In a hypomorphic janus mutant, the activity of DCL1, the numbers of MC, and the interaction of primary miRNA transcript (pri-miRNAs) with MC are reduced. These data suggest that JANUS promotes the assembly and activity of MC through its interaction with MC and/or pri-miRNAs. In addition, JANUS modulates the transcription of some pri-miRNAs as it binds the promoter of pri-miRNAs and promotes Pol II occupancy of at their promoters. Moreover, global splicing defects are detected in janus. Taken together, our study reveals a novel role of a conserved splicing factor in miRNA biogenesis.

2023-06-01 | GSE229519 | GEO

Janus sp. 1 GYN-2021a

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Janus sp. 2 GYN-2021a

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2019-05-18 | GSE131410 | GEO

Trigeminal nerve derived-substance P regulates biological characteristics of limbal stem cells through PI3K-AKT signaling pathway

Project description:To investigate the function of Substance P- NK1R interaction in the regulation of limbal stem cells, we subconjunctivally injected NK1 receptor antagonist L-733,060 on mice. By RNA sequencing, we found that antagonist injection significantly downregulated PI3K-AKT signaling related genes level. Collectively, our work reveals that Substance P- NK1R interaction is a novel player in limbal stem cells regulation.

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Janus kinase-Inhibition modulates hepatitis E virus infection

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