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TranscriptomicsSmall RNA profiling in Drosophila wild-type and nbr[f02257] mutants
nbr/CG9247 gene regulates 3'end heterogeneity of a subset of miRNAs. It is not clear how broad this effect is on small RNA population. To address this, we compared small RNA population in wild-type and tmr[f02257] mutants. This approach identified more miRNAs whose 3'end heterogeneity was affected i...
ORGANISM(S): Drosophila melanogaster 
2011-11-01 | E-GEOD-32684 | biostudies-arrayexpress
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TranscriptomicsAgo1 vs Ago2-IP small RNA deep-sequencing with age in Drosophila
Drosophila miRNAs show distinct change in isoform distribution pattern with age. Some miRNAs show accumul...
...immunoprecipitated by anti-Ago1 and anti-FLAG M2 beads respectively. RNA was purified from the beads, P32-labeled, and small RNA fraction was gel-purififed. Small RNA libraries were prepared using Illumina's TruSeq small RNA sample preparation kit (#RS-200-0012, Illumina, Inc. San Diego, CA), following the manufacturer's protocol. The libraries were multiplexed and sequenced on HiSeq2000 platform (Illumina).
ORGANISM(S): Drosophila melanogaster 
2014-01-01 | E-GEOD-52961 | biostudies-arrayexpress
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Transcriptomicssmall RNA deep-sequencing with oxidation/β-elimination with age in Drosophila
Drosophila miRNAs show distinct change in isoform distribution pattern with age. Some miRNAs show accumulation of the short isoforms, while other miRNAs show the accumulation of the long isoforms with age. The increase of the long isoforms of some miRNAs reflects increased 2'-O-methylated miRNA isofo...
ORGANISM(S): Drosophila melanogaster 
2014-01-01 | E-GEOD-52960 | biostudies-arrayexpress
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ProteomicsMudPIT analyses of the proteins co-purified with the Interactor of Little Elongation Complex ELL subunit 1 (FLAG-ICE1), WT MED26, and D2G8 NTD-mutant MED26, affinity-purified from HEK293T cell lines
Protein Complexes Purification: We generated 293FRT cell lines that stably express FLAG-tagged ICE1 (Interactor of Little Elongation Complex ELL subunit 1; KIAA0947). We extracted nuclear proteins in the presence of Benzonase to digest nucleic acids, including both DNA and RNA, and purified the litt...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-02-20 | MSV000083465 | MassIVE
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TranscriptomicsGene expression profiles in dorsal skin of hairless mice orally administrated collagen hydrolysate for 12 weeks
Dietary collagen hydrolysate has been conjectured to improve skin barrier function. To investigate the effect of long-term collagen hydrolysate administration on the skin, we evaluated stratum corneum water content and skin elasticity in intrinsic aged mice. Female 9-week-old hairless mice were fed a...
ORGANISM(S): Mus musculus 
2015-06-12 | E-GEOD-62649 | biostudies-arrayexpress
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