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ProteomicsA novel pathway for inositol conversion into propionate by Anaerostipes supports its potential health impact.
A novel propionate production pathway from inositol by commensal Anaerostipes which is known as intestinal butyrate producer was studied. The physiological relevance of this pathway was explored in human gut as well as potential health impact of Anaerostipes species to the human host.
ORGANISM(S): Anaerostipes Rhamnosivorans 
2021-07-05 | PXD021084 | Pride
Human microbiome Inositol Propionate Anaerostipes 
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ProteomicsElectron Transfer/ Higher Energy Collisional Dissociation of Doubly Charged Peptide Ions: Identification of Labile Protein Phosphorylations
Fragmentation behaviour of labile phosphorylated peptides
ORGANISM(S): Bos Taurus (bovine) Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast) 
2019-05-27 | PXD012989 | Pride
Labile phosphorylations Ethcd 
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ProteomicsAn Uncommon Phosphorylation Mode Regulates the Activity and Protein Interactions of N-Acetylglucosamine Kinase
Reversible protein phosphorylation serves as a pivotal signaling mechanism in eukaryotic cells, contrasting with the poorly understood protein pyrophosphorylation, a posttranslational modification (PTM) whose functions in living organisms remain elusive. Unlike kinase-mediated protein phosphorylation...
ORGANISM(S): Homo Sapiens (human) 
2024-05-22 | PXD049339 | Pride
Phosphorylation Pulldown Lfq Affinity-purification ms Pyrophosphorylation 
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ProteomicsPyrophosphoproteomics in HCT116 wildtype vs PPIP5K1/2 knockout
In vitro, both 5PP-InsP5 (IP7) and 1,5(PP)2InsP4 (IP8) can pyrophosphorylate peptides and proteins. It is unclear, which inositol pyrophosphate species is responsible for installing endogenous pyrophosphorylation in cells. In order to test this, we used HCT116 cells deficient in IP8 synthesis. Both e...
ORGANISM(S): Homo Sapiens (human) 
2024-03-12 | PXD048031 | JPOST Repository
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ProteomicsPyrophosphoproteomics on NOLC1 / TCOF1 / IWS1 pulldown samples
Known pyrophosphorylation substrates NOLC1, TCOF1, IWS1 were overexpressed in IP6K1-/- double KO HEK293T cells, along with active WT or kinase-dead IP6K1 i.e. background high or low in inositol pyrophosphates. the three proteins were pulled down via their SFB-tag and submitted to a pyrophosphoproteom...
ORGANISM(S): Homo Sapiens (human) 
2024-03-12 | PXD038962 | JPOST Repository
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ProteomicsPyrophosphoproteomics: extensive protein pyrophosphorylation revealed in human cell lines
Reversible protein phosphorylation is a central signaling mechanism in eukaryotic cells. While the identification of canonical phosphorylation sites using mass-spectrometry (MS) based proteomics has become routine, annotation of non-canonical phosphorylation has remained a challenge. Here, we report ...
ORGANISM(S): Homo Sapiens (human) 
2024-03-12 | PXD038963 | JPOST Repository
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