{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(15)"],"submitter":["Bongiorno G"],"pubmed_abstract":["<i>Phytophthora capsici</i> causes destructive disease in several crop species, including pepper (<i>Capsicum annuum</i> L.). Resistance in this species is physiologically and genetically complex due to many <i>P. capsici</i> virulence phenotypes and different QTLs and R genes among the identified resistance sources. Several primer pairs were designed to follow an SNP (G/A) within the <i>CA_011264</i> locus linked to the <i>Pc5.1</i> locus. All primer pairs were designed on DNA sequences derived from <i>CaDMR1</i>, a homoserine kinase (HSK), which is a gene candidate responsible for the major QTL on chromosome <i>P5</i> for resistance to <i>P. capsici</i>. A panel of 69 pepper genotypes from the Southern Seed germplasm collection was used to screen the primer pairs designed. Of these, two primers (<i>Phyto</i>_for_2 and <i>Phyto</i>_rev_2) surrounding the SNP proved successful in discriminating susceptible and resistant genotypes when combined with a restriction enzyme (<i>BtgI</i>). This new marker (called <i>Phyto</i>) worked as expected in all genotypes tested, proving to be an excellent candidate for marker-assisted selection in breeding programs aimed at introgressing the resistant locus into pure lines."],"journal":["Plants (Basel, Switzerland)"],"pagination":["2757"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10421461"],"repository":["biostudies-literature"],"pubmed_title":["Development and Application of a Cleaved Amplified Polymorphic Sequence Marker (<i>Phyto</i>) Linked to the <i>Pc5.1</i> Locus Conferring Resistance to <i>Phytophthora capsici</i> in Pepper (<i>Capsicum annuum</i> L.)."],"pmcid":["PMC10421461"],"pubmed_authors":["Marconi G","Di Noia A","Ciancaleoni S","Albertini E","Cassibba V","Bongiorno G"],"additional_accession":[]},"is_claimable":false,"name":"Development and Application of a Cleaved Amplified Polymorphic Sequence Marker (<i>Phyto</i>) Linked to the <i>Pc5.1</i> Locus Conferring Resistance to <i>Phytophthora capsici</i> in Pepper (<i>Capsicum annuum</i> L.).","description":"<i>Phytophthora capsici</i> causes destructive disease in several crop species, including pepper (<i>Capsicum annuum</i> L.). Resistance in this species is physiologically and genetically complex due to many <i>P. capsici</i> virulence phenotypes and different QTLs and R genes among the identified resistance sources. Several primer pairs were designed to follow an SNP (G/A) within the <i>CA_011264</i> locus linked to the <i>Pc5.1</i> locus. All primer pairs were designed on DNA sequences derived from <i>CaDMR1</i>, a homoserine kinase (HSK), which is a gene candidate responsible for the major QTL on chromosome <i>P5</i> for resistance to <i>P. capsici</i>. A panel of 69 pepper genotypes from the Southern Seed germplasm collection was used to screen the primer pairs designed. Of these, two primers (<i>Phyto</i>_for_2 and <i>Phyto</i>_rev_2) surrounding the SNP proved successful in discriminating susceptible and resistant genotypes when combined with a restriction enzyme (<i>BtgI</i>). This new marker (called <i>Phyto</i>) worked as expected in all genotypes tested, proving to be an excellent candidate for marker-assisted selection in breeding programs aimed at introgressing the resistant locus into pure lines.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jul","modification":"2024-11-13T19:09:56.951Z","creation":"2024-11-13T19:09:56.951Z"},"accession":"S-EPMC10421461","cross_references":{"pubmed":["37570909"],"doi":["10.3390/plants12152757"]}}