{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(1)"],"submitter":["Jayakumar R"],"pubmed_abstract":["<h4>Background</h4>The diverse specificity mode of cancer treatment targets and chemo resistance demands the necessity of drug entities which can address the devastating dynamicity of the disease.<h4>Objectives</h4>To check the anti-tumour potential of traditional medicine rich in polyherbal components and metal nanoparticle namely Arkeshwara rasa (AR).<h4>Material methods</h4>The AR was prepared in a modified version with reference from Rasaratna Samuchaya and characterized using sophisticated instrumental analysis including XRD, SEM-EDAX, TEM, TGA-DSC, and LC-MS and tested against the MDA-MB-231 cell line to screen cell viability and the cytotoxicity with MTT, SRB and the AO assay.<h4>Results</h4>XRD pattern shows cubic tetrahedrite structure with Sb, Cu, S peaks and trace elements like Fe, Mg, etc. The particle size of AR ranges between 20 and 30 nm. The TGA points thermal decomposition at 210 °C and the metal sulphide peaks in DSC. LC-MS analysis reveals the components of the formulation more on the flavonoid portion. The IC<sub>50</sub> value of MTT and SRB are 25.28 μg/mL and 31.7 μg/mL respectively. The AO colorimeter substantiated the cell viability and the apoptosis figures of the same cell line. The AR exhibits cytotoxicity and reaffirms the apoptosis fraction with SRB assay.<h4>Conclusions</h4>The Hesperidine, Neohesperidin, Rutin components in the phytochemical pool can synergize the anti-tumour potential with either influencing cellular pathways or decreasing chemo resistance to conventional treatment. AR need to be further experimented with reverse transcription, flow cytometry, western blotting, etc."],"journal":["Journal of Ayurveda and integrative medicine"],"pagination":["100823"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10792653"],"repository":["biostudies-literature"],"pubmed_title":["Pharmaceutical characterization and exploration of Arkeshwara rasa in MDA-MB-231 cells."],"pmcid":["PMC10792653"],"pubmed_authors":["Joshi N","Cholke K","Sharma S","Fatima Z","Kumar P","Pandey A","Trigun SK","Jayakumar R","Dash MK","Gulati S"],"additional_accession":[]},"is_claimable":false,"name":"Pharmaceutical characterization and exploration of Arkeshwara rasa in MDA-MB-231 cells.","description":"<h4>Background</h4>The diverse specificity mode of cancer treatment targets and chemo resistance demands the necessity of drug entities which can address the devastating dynamicity of the disease.<h4>Objectives</h4>To check the anti-tumour potential of traditional medicine rich in polyherbal components and metal nanoparticle namely Arkeshwara rasa (AR).<h4>Material methods</h4>The AR was prepared in a modified version with reference from Rasaratna Samuchaya and characterized using sophisticated instrumental analysis including XRD, SEM-EDAX, TEM, TGA-DSC, and LC-MS and tested against the MDA-MB-231 cell line to screen cell viability and the cytotoxicity with MTT, SRB and the AO assay.<h4>Results</h4>XRD pattern shows cubic tetrahedrite structure with Sb, Cu, S peaks and trace elements like Fe, Mg, etc. The particle size of AR ranges between 20 and 30 nm. The TGA points thermal decomposition at 210 °C and the metal sulphide peaks in DSC. LC-MS analysis reveals the components of the formulation more on the flavonoid portion. The IC<sub>50</sub> value of MTT and SRB are 25.28 μg/mL and 31.7 μg/mL respectively. The AO colorimeter substantiated the cell viability and the apoptosis figures of the same cell line. The AR exhibits cytotoxicity and reaffirms the apoptosis fraction with SRB assay.<h4>Conclusions</h4>The Hesperidine, Neohesperidin, Rutin components in the phytochemical pool can synergize the anti-tumour potential with either influencing cellular pathways or decreasing chemo resistance to conventional treatment. AR need to be further experimented with reverse transcription, flow cytometry, western blotting, etc.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Dec","modification":"2024-10-17T19:29:21.81Z","creation":"2024-10-17T19:29:21.81Z"},"accession":"S-EPMC10792653","cross_references":{"pubmed":["38160612"],"doi":["10.1016/j.jaim.2023.100823"]}}