{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shah K"],"funding":["U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences","U.S. Department of Health & Human Services | NIH | National Cancer Institute","U.S. Department of Defense","NIGMS NIH HHS"],"pagination":["3159"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11009413"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["How RNA-binding proteins (RBPs) convey regulatory instructions to the core effectors of RNA processing is unclear. Here, we document the existence and functions of a multivalent RBP-effector interface. We show that the effector interface of a conserved RBP with an essential role in metazoan development, Unkempt, is mediated by a novel type of 'dual-purpose' peptide motifs that can contact two different surfaces of interacting proteins. Unexpectedly, we find that the multivalent contacts do not merely serve effector recruitment but are required for the accuracy of RNA recognition by Unkempt. Systems analyses reveal that multivalent RBP-effector contacts can repurpose the principal activity of an effector for a different function, as we demonstrate for the reuse of the central eukaryotic mRNA decay factor CCR4-NOT in translational control. Our study establishes the molecular assembly and functional principles of an RBP-effector interface."],"journal":["Nature communications"],"pubmed_title":["Regulation by the RNA-binding protein Unkempt at its effector interface."],"pmcid":["PMC11009413"],"funding_grant_id":["R35 GM151004","W81XWH-17-1-0082","Intramural Research Program, Center for Cancer Research","R35 GM142864","R01 GM144693"],"pubmed_authors":["Turner DJ","He S","Valkov E","Murn J","Dominguez D","Igreja C","Corbo J","Bateman JM","Shah K","Rebbani K","Cheloufi S"],"additional_accession":[]},"is_claimable":false,"name":"Regulation by the RNA-binding protein Unkempt at its effector interface.","description":"How RNA-binding proteins (RBPs) convey regulatory instructions to the core effectors of RNA processing is unclear. Here, we document the existence and functions of a multivalent RBP-effector interface. We show that the effector interface of a conserved RBP with an essential role in metazoan development, Unkempt, is mediated by a novel type of 'dual-purpose' peptide motifs that can contact two different surfaces of interacting proteins. Unexpectedly, we find that the multivalent contacts do not merely serve effector recruitment but are required for the accuracy of RNA recognition by Unkempt. Systems analyses reveal that multivalent RBP-effector contacts can repurpose the principal activity of an effector for a different function, as we demonstrate for the reuse of the central eukaryotic mRNA decay factor CCR4-NOT in translational control. Our study establishes the molecular assembly and functional principles of an RBP-effector interface.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2024-10-16T13:59:18.062Z","creation":"2024-10-16T13:59:18.062Z"},"accession":"S-EPMC11009413","cross_references":{"pubmed":["38605040"],"doi":["10.1038/s41467-024-47449-4"]}}