{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Li X"],"pubmed_abstract":["In biological research, qPCR is a technique that is frequently used to measure gene expression levels. The calculation of gene amplification efficiency is a critical step in the processing of qPCR data since it helps to decide which method to employ to compute gene expression levels. Here, we introduce the R package qPCRtools, which enables users to analyze the efficiency of gene amplification. Additionally, this software can determine gene expression levels using one of three approaches: the conventional curve-based method, the 2<sup>-ΔΔCt</sup> method, and the SATQPCR method. The qPCRtools package produces a table with the statistical data of each method as well as a figure with a box or bar plot illustrating the results. The R package qPCRtools is freely available at CRAN (https://CRAN.R-project.org/package=qPCRtools) or GitHub (https://github.com/lixiang117423/qPCRtools/tree/main/CRAN/qPCRtools)."],"journal":["Frontiers in genetics"],"pagination":["1002704"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9513427"],"repository":["biostudies-literature"],"pubmed_title":["qPCRtools: An R package for qPCR data processing and visualization."],"pmcid":["PMC9513427"],"pubmed_authors":["Li X","Liu Y","Li J","Mei X","Huang H","Wang Y"],"additional_accession":[]},"is_claimable":false,"name":"qPCRtools: An R package for qPCR data processing and visualization.","description":"In biological research, qPCR is a technique that is frequently used to measure gene expression levels. The calculation of gene amplification efficiency is a critical step in the processing of qPCR data since it helps to decide which method to employ to compute gene expression levels. Here, we introduce the R package qPCRtools, which enables users to analyze the efficiency of gene amplification. Additionally, this software can determine gene expression levels using one of three approaches: the conventional curve-based method, the 2<sup>-ΔΔCt</sup> method, and the SATQPCR method. The qPCRtools package produces a table with the statistical data of each method as well as a figure with a box or bar plot illustrating the results. The R package qPCRtools is freely available at CRAN (https://CRAN.R-project.org/package=qPCRtools) or GitHub (https://github.com/lixiang117423/qPCRtools/tree/main/CRAN/qPCRtools).","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2024-12-04T03:28:26.161Z","creation":"2024-12-04T03:28:26.161Z"},"accession":"S-EPMC9513427","cross_references":{"pubmed":["36176301"],"doi":["10.3389/fgene.2022.1002704"]}}