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Transcription profiling by array of human HhAntag-treated pancreatic xenograft tumors


ABSTRACT: Four vehicle-treated and four HhAntag-treated pancreatic xenograft tumors were profiled for gene expression changes using Affymetrix U133 Plus 2.0 and Affymetrix Mouse Genome 430 2.0 arrays. Keywords: comparative gene expression, hedgehog, hh A primary human pancreatic tumor xenograft (1051178-A) was established by direct implantation of surgical material into female CD1 nu/nu mice of 6-8 weeks of age. Tumors were serially passaged into larger cohorts of mice for efficacy testing and subsequently distributed into tumor volume-matched cohorts upon tumors reaching between 200 to 350 mm3. HhAntag was resuspended in 0.5% methyl-cellulose, 0.2% Tween-80 (MCT) and administered orally twice daily at 75 mg/kg from a 10 mg/ml suspension. MCT alone served as vehicle control. Tumor xenografts (4/group) were excised following 21 days of dosing and RNA was extracted. Preparation of complementary RNA, Human Genome U133 Plus 2.0 array and Mouse Genome 430 2.0 array hybridizations, and subsequent data analysis were carried out using Affymetrix protocols, with signal intensities being determined by the MAS5.0 algorithm.

OTHER RELATED OMICS DATASETS IN: PRJNA105863

PROVIDER: E-GEOD-11981 | ExpressionAtlas | 2020-07-28

REPOSITORIES: ExpressionAtlas

Dataset's files

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Action DRS
E-GEOD-11981-analysis-methods.tsv Tabular
E-GEOD-11981-atlasExperimentSummary.Rdata Rdata
E-GEOD-11981-configuration.xml Xml
E-GEOD-11981-percentile-ranks.tsv Tabular
E-GEOD-11981.condensed-sdrf.tsv Tabular
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Publications


Ligand-dependent activation of the hedgehog (Hh) signalling pathway has been associated with tumorigenesis in a number of human tissues. Here we show that, although previous reports have described a cell-autonomous role for Hh signalling in these tumours, Hh ligands fail to activate signalling in tumour epithelial cells. In contrast, our data support ligand-dependent activation of the Hh pathway in the stromal microenvironment. Specific inhibition of Hh signalling using small molecule inhibitors  ...[more]

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