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Whole-Tissue Gene Expression Study of Pancreatic Ductal Adenocarcinoma

ABSTRACT: Expression analysis of 36 pancreatic ductal adenocarcinoma tumors and matching normal pancreatic tissue samples from pancreatic cancer patients of the Clinical Institute Fundeni (ICF) using Affymetrix U133 Plus 2.0 whole-genome chips. Pairs of normal and tumor tissue samples were obtained at the time of surgery from resected pancreas of 36 pancreatic cancer patients. Gene expression was analyzed on Affymetrix U133 plus 2.0 whole genome microarrays. For three of the 36 normal-tumor sample pairs we carried out replicate microarray hybridizations in order to gauge the technical measurement errors. We thus performed 78 genechip hybridizations in total. A patient sample pair was excluded from further analysis since one of the samples did not meet the quality controls. The microarray data was subsequently normalized using the RMA algorithm.

ORGANISM(S): Homo sapiens

SUBMITTER: Liviu Badea 

PROVIDER: E-GEOD-15471 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Combined gene expression analysis of whole-tissue and microdissected pancreatic ductal adenocarcinoma identifies genes specifically overexpressed in tumor epithelia.

Badea Liviu L   Herlea Vlad V   Dima Simona Olimpia SO   Dumitrascu Traian T   Popescu Irinel I  

Hepato-gastroenterology 20081101 88

<h4>Background/aims</h4>The precise details of pancreatic ductal adenocarcinoma (PDAC) pathogenesis are still insufficiently known, requiring the use of high-throughput methods. However, PDAC is especially difficult to study using microarrays due to its strong desmoplastic reaction, which involves a hyperproliferating stroma that effectively "masks" the contribution of the minoritary neoplastic epithelial cells. Thus it is not clear which of the genes that have been found differentially expresse  ...[more]

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