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E-GEOD-15205

ABSTRACT: Gene expression profile of ARPE-19 cells treated with TGF and TNF for 6 and 42 hour.

PROVIDER: E-GEOD-15205 | ExpressionAtlas | 2025-01-24

REPOSITORIES: ExpressionAtlas

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Dataset's files

Source:

			Action	DRS
	E-GEOD-15205-analysis-methods.tsv	Tabular
	E-GEOD-15205-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-15205-configuration.xml	Xml
	E-GEOD-15205-percentile-ranks.tsv	Tabular
	E-GEOD-15205.condensed-sdrf.tsv	Tabular

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Transcription profiling of human retinal pigment epithelium (RPE) cell line (ARPE-19) in response to combination treatment of TGF and TNF

Project description:The purpose of this study is to determine the changes in gene expression by a human retinal pigment epithelium (RPE) cell line (ARPE-19) in response to combination treatment of TGF and TNF, which induces phenotypic changes in vitro that mimic the EMT (Epithelial-to-Mesenchymal Transition). For this purpose, total RNA was extracted from TGF and TNF-treated ARPE-19 cells and differential gene expression between each time point (0, 1, 6, 16, 24, 42, and 60 hours) was determined using genechip arrays (Affymetrix, Human Genome U133). Experiment Overall Design: ARPE19 cell lines treated with TGF and TNF for 0, 1, 6, 16, 24, 42, and 60 hour. Each experiment were repeated three times. But 1 hour experiment was repeated two times.

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