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Transcription profiling of human retinal pigment epithelium (RPE) cell line (ARPE-19) in response to combination treatment of TGF and TNF

ABSTRACT: The purpose of this study is to determine the changes in gene expression by a human retinal pigment epithelium (RPE) cell line (ARPE-19) in response to combination treatment of TGF and TNF, which induces phenotypic changes in vitro that mimic the EMT (Epithelial-to-Mesenchymal Transition). For this purpose, total RNA was extracted from TGF and TNF-treated ARPE-19 cells and differential gene expression between each time point (0, 1, 6, 16, 24, 42, and 60 hours) was determined using genechip arrays (Affymetrix, Human Genome U133). Experiment Overall Design: ARPE19 cell lines treated with TGF and TNF for 0, 1, 6, 16, 24, 42, and 60 hour. Each experiment were repeated three times. But 1 hour experiment was repeated two times.

ORGANISM(S): Homo sapiens

SUBMITTER: Rui Ose 

PROVIDER: E-GEOD-12548 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Tumor necrosis factor-alpha regulates transforming growth factor-beta-dependent epithelial-mesenchymal transition by promoting hyaluronan-CD44-moesin interaction.

Takahashi Eri E   Nagano Osamu O   Ishimoto Takatsugu T   Yae Toshifumi T   Suzuki Yoshimi Y   Shinoda Takeshi T   Nakamura Satoshi S   Niwa Shinichiro S   Ikeda Shun S   Koga Hisashi H   Tanihara Hidenobu H   Saya Hideyuki H  

The Journal of biological chemistry 20091204 6

Aberrant epithelial-mesenchymal transition (EMT) is involved in development of fibrotic disorders and cancer invasion. Alterations of cell-extracellular matrix interaction also contribute to those pathological conditions. However, the functional interplay between EMT and cell-extracellular matrix interactions remains poorly understood. We now show that the inflammatory mediator tumor necrosis factor-alpha (TNF-alpha) induces the formation of fibrotic foci by cultured retinal pigment epithelial c  ...[more]

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