Project description:This SuperSeries is composed of the following subset Series:; GSE15466: Transcriptional response of Drosophila cells to FHV infection (infection experiment); GSE15467: Transcriptional response of Drosophila cells to FHV RNA1 replicon expression (replicon experiment) Experiment Overall Design: Refer to individual Series
Project description:Innate immune priming increases an organism’s survival of a second infection after an initial, non-lethal infection. We used Drosophila melanogaster and an insect-derived strain of Enterococcus faecalis to study transcriptional control of priming. In contrast to other pathogens, the enhanced survival in primed animals does not correlate with decreased E. faecalis load. Further analysis shows that primed organisms tolerate, rather than resist infection. Using RNA-seq of immune tissues, we found many genes were upregulated in only primed flies, suggesting a distinct transcriptional program in response to initial and secondary infections. In contrast, few genes continuously express throughout the experiment or more efficiently re-activate upon reinfection. Priming experiments in immune deficient mutants revealed Imd is largely dispensable for responding to a single infection but needed to fully prime. Together, this indicates the fly’s innate immune response is plastic — differing in immune strategy, transcriptional program, and pathway use depending on infection history.
Project description:We performed an mRNA-sequencing experiment using Drosophila midgut to find JHDM2-dependent signaling pathways in response to bacterial challenge and downstream target genes that are epigenetically regulated across genome. The sequenced reads from Illumina Hiseq2000 that passed quality filters were mapped to Drosophila genome (dm3) using Tophat and then quantitatively analyzed by Cufflinks at the gene level. By comparing Wild type and JHDM2 knock-down samples post-infection with Ecc15, we profiled JHDM2-dependent gene expression changes induced by Ecc15 infection.
Project description:Bovine tuberculosis, caused by Mycobacterium bovis, is a disease of considerable economic importance yet comparatively little is known about the bovine immune response to the disease. Alveolar macrophages are one of the first cells to encounter mycobacteria following infection. In this experiment we investigated the early transcriptional response of bovine alveolar macrophages following infection with M. bovis. The transcriptional response to heat-killed M. bovis was also investigated to look for genes that are only differentially transcribed in response to the live organism.