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E-GEOD-17610

ABSTRACT: Transcription profiling by array of Arabidopsis mutant for agl15 and agl18 or overexpressing AGL15 during somatic embryogenesis

OTHER RELATED OMICS DATASETS IN: GSE17742PRJNA123281PRJNA118373PRJNA123365

PROVIDER: E-GEOD-17610 | ExpressionAtlas | 2014-07-03

REPOSITORIES: ExpressionAtlas

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Dataset's files

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			Action	DRS
	E-GEOD-17610-analysis-methods.tsv	Tabular
	E-GEOD-17610-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-17610-configuration.xml	Xml
	E-GEOD-17610-percentile-ranks.tsv	Tabular
	E-GEOD-17610-zoomifications-log.tsv	Tabular

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Gene expression in response to AGL15 during somatic embryogenesis

Project description:Transcript accumulation was measured using the Affymetrix Arabidopsis ATH1 Genome Array [ATH1-121501] to document changes in response to the MADS-domain transcription factor AGAMOUS-Like 15 during somatic embryogenesis. A somatic embryo system was used where mature seed is allowed to complete germination in liquid MS media containing 2,4-D and seedlings produce somatic embryos from the shoot apical meristem (SAM) region. The frequency with which these embryos are produced directly correlates with AGL15 accumulation. Experiment Overall Design: Wild type Arabidopsis (Columbia ecotype) with normal amounts of AGL15 were compared to (1) 35S:AGL15 that constitutively expresses AGL15 and produces more somatic embryos from the shoot apical region than wild type and (2) agl15 agl18 double loss-of-function mutants that produce less somatic embryos from the shoot apical region than wild type.

2009-09-25 | E-GEOD-17610 | biostudies-arrayexpress

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