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E-GEOD-20621

ABSTRACT: Transcription profiling by array of Mus musculus colonic mucosa after treatment with dextran sodium sulphate (DSS) for 2 or 7 days which induces experimental colitis

OTHER RELATED OMICS DATASETS IN: PRJNA124807

PROVIDER: E-GEOD-20621 | ExpressionAtlas | 2015-01-07

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-20621-analysis-methods.tsv	Tabular
	E-GEOD-20621-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-20621-configuration.xml	Xml
	E-GEOD-20621-configuration.xml.beforeQC	Xml
	E-GEOD-20621-percentile-ranks.tsv	Tabular

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Immunoregulatory actions of epithelial cell PPAR g at the colonic mucosa

2010-07-08 | E-GEOD-20621 | biostudies-arrayexpress

Colonic gene expression data of acute and chronic dextran sodium sulphate (DSS) colitis mice

2013-09-03 | GSE42768 | GEO

E-GEOD-34874

Project description:Transcription profiling by array of murine colon tissue after exposure to 4% dextran sodium sulphate (DSS) for 6 days followed by 4 days of water or 1% L-arginine to study the effect of L-arginine on DSS-induced colitis

2015-01-07 | E-GEOD-34874 | ExpressionAtlas

Transcription profiling of rat dextran sulphate sodium-induced colitis model

Project description:Background & Aims: Dextran sulphate sodium (DSS) induced colitis in rats is one of the most widely used models of inflammatory bowel disease. Animal models can provide new insights into the pathogenesis of intestinal inflammation, which is still unknown. We have performed a genomic analysis of the DSS rat colitis including an acute and a recovery phase. Methods: Expression profile of 6 control rats were compared with colitic rats at day 1 every other day until day 23 after DSS treatment using the GeneChip Rat Genome 230 2.0 Array (Affymetrix). Functional and pathways analysis were made with the differentially expressed genes. Experiment Overall Design: Experimental design: DSS was administered to animals in drinking water as follows:5%DSS from day 1 to 7, 3%DSS from day 8 to 15, 0% DSS from day 16 to 23. Samples were recovered at days 1, 3, 5, 7 (5%DSS), 9, 11, 13, 15 (2%DSS), 17, 19, 21 and 23 (0%DSS). According to inflammatory markers (Myeloperoxidase activity activity, body weight loss, colonic weigth/length ratio), three replicates at each time point were selected for genomic analysis and 6 control healthy rats (42 arrays). Experiment Overall Design: RNA was extracted from homogenized full-thickness colonic tissues in TrizolÂ® reagent (Invitrogen) and purified with RNeasy affinity columns (Qiagen), according to manufacturerÂ´s protocol. The microarray analysis was performed by Progenika Biopharma (Bilbao, Spain) on GeneChipÂ® Rat Genome 230 2.0 Array (Affymetrix). All sample labeling (biotin), hybridization, staining and scanning procedures were carried out using Affimetrix, standard protocols (www.affymetrix.com). Normalization was carried out using Bioconductor sofware (affyPLM package).

2008-11-13 | E-GEOD-9281 | biostudies-arrayexpress

Immunoregulatory actions of T cell PPAR g at the colonic mucosa

Project description:BACKGROUND: Peroxisome proliferator-activated receptor g (PPAR g) is a nuclear receptor whose activation has been shown to modulate macrophage and epithelial cell-mediated inflammation. The objective of this study was to use a systems approach for investigating the mechanism by which the deletion of PPAR g in T cells modulates the severity of dextran-sodium sulfate (DSS)-induced colitis, immune cell distribution and global gene expression. METHODS: Wild-type (WT) or PPAR g flfl; CD4 Cre+ (CD4cre) mice in a C57BL/6 background were challenged with 2.5% DSS in their drinking water for 0, 2, or 7 days. Mice were scored on disease severity both clinically and histopathologically. Flow cytometry was used to assess lymphocyte and macrophage populations in the blood, spleen, and mesenteric lymph nodes (MLN). Global gene expression in colonic mucosa was profiled using Affymetrix microarrays. RESULTS: Both disease severity and inflammation-related body weight loss were accelerated by the deficiency of PPAR g in T cells. Examination of colon histopathology revealed significantly greater epithelial erosion, leukocyte infiltration, and mucosal thickening in the CD4cre mice on day 7. CD4cre mice had more CD8+ T cells than wt mice and fewer CD4+FoxP3+ regulatory T cells (Treg) and IL10+CD4+ T cells in blood and MLN, respectively. Transcriptomic profiling revealed around 3000 genes being transcriptionally altered as a result of DSS challenge in CD4cre mice. These included up-regulated adhesion molecules on day 7 and proinflammatory cytokines interleukin-6 (IL-6) and IL-1b, and suppressor of cytokine signaling 3 (SOCS-3) mRNA expression. CONCLUSIONS: These findings suggest that T cell PPAR g down-regulates inflammation during DSS colitis by inhibiting colonic expression of inflammatory mediators and increasing MLN Treg. Colonic mucosa from wt and CD4cre mice were sampled at 0 (no DSS), 2, and 7 days of DSS-induced experimental colitis

2010-07-08 | E-GEOD-20523 | biostudies-arrayexpress

E-GEOD-42768

Project description:Transcription profiling by array of colon tissues isolated from mice with acute and chronic dextran sodium sulphate (DSS)-induced colitis

2014-10-17 | E-GEOD-42768 | ExpressionAtlas

Colonic gene expression data of acute and chronic dextran sodium sulphate (DSS) colitis mice

Project description:The lack of suitable animal models reflecting chronically relapsing inflammation and tissue remodeling have hindered fibrosis research in inflammatory bowel diseases (IBD). This study investigated changes in connective tissue in a chronic murine model using different cycles of dextran sodium sulphate (DSS) to mimic the relapsing nature of the disease. We used whole gene expression arrays to study differences in colonic gene expression levels between acute and more chronic DSS colitis, Acute and chronic relapsing colonic inflammation was induced in C57BL6 female mice using several cycles of exposure to DSS in drinking water, followed by recovery phases. Total RNA, extracted from snap frozen colon from five mice per condition was used to analyze mRNA expression via Affymetrix Mouse Gene 1.0 ST arrays.

2013-09-03 | E-GEOD-42768 | biostudies-arrayexpress

Dextran sodium sulfate treatment of wild type and Pirc rats alters colonic normal tissue gene expression

Project description:Dextran sodium sulfate (DSS) causes inflammation in the gut similar to ulcerative colitis in humans. Patients with ulcerative colitis have increased risk of developing colon cancer. We sought to determine which genes are altered in the normal colonic epithelium, and which changes depend on the Pirc mutation. 97 day old (ACIxF344)F1 wild type and Pirc male rats either untreated or given 4% DSS in the drinking water from 40-47 and 54-61 days of age, housed in 12 hour light:12 dark, ad lib feeding. Normal colonic tissue was collected from the distal colon at 97 days of age.

2014-01-14 | E-GEOD-54005 | biostudies-arrayexpress

Transcriptional profiling of murine distal colon from C57BL/6 (WT), Il20KO, Il20raKO and Il20rbKO mice during acute DSS colitis

Project description:Experimental colitis was induced in mice by the administration of 1.5% (w/v) Dextran sulfate sodium salt (DSS, colitis grade, 36-50kDa, MP Biomedicals) in the drinking water for 7 days followed by normal drinking water w/o DSS. Distal colons were collected two days later.

2023-10-01 | E-MTAB-12737 | biostudies-arrayexpress

Transcriptional profiling of murine distal colon from C57BL/6 (WT) and Stat2KO mice during acute DSS colitis.

Project description:Experimental colitis was induced in mice by the administration of 2% (w/v) Dextran sulfate sodium salt (DSS, colitis grade, 36-50kDa, MP Biomedicals) in the drinking water for 7 days followed by normal drinking water w/o DSS. Distal colons were collected two days later.

2023-10-01 | E-MTAB-12655 | biostudies-arrayexpress

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