E-GEOD-2565
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: PRJNA91969
PROVIDER: E-GEOD-2565 | ExpressionAtlas | 2015-09-18
REPOSITORIES: ExpressionAtlas
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| E-GEOD-2565-analysis-methods.tsv | Tabular | |||
| E-GEOD-2565-atlasExperimentSummary.Rdata | Rdata | |||
| E-GEOD-2565-configuration.xml | Xml | |||
| E-GEOD-2565-percentile-ranks.tsv | Tabular | |||
| E-GEOD-2565.condensed-sdrf.tsv | Tabular |
Items per page: 5 1 - 5 of 301 |
Similar Datasets
Project description:Transcription profiling of Drosophila exposed to a levitation magnet for different lengths of time
2015-10-22 | E-MEXP-2082 | ExpressionAtlas
Project description:Carbonyl chloride (phosgene) is a toxic industrial compound (TIC) widely used in industry for the production of synthetic products, such as polyfoam rubber, plastics, and dyes. Exposure to phosgene results in a latent (1-24 hr), potentially life-threatening pulmonary edema and irreversible acute lung injury. A genomic approach was utilized to investigate the molecular mechanism of phosgene-induced lung injury. CD-1 male mice were exposed whole-body to either air or a concentration x time (c x t) amount of 32 mg/m3 (8 ppm) phosgene for 20 min (640 mg x min/m3). Lung tissue was collected from air- or phosgene-exposed mice at 0.5, 1, 4, 8, 12, 24, 48, and 72 hr post-exposure. RNA was extracted from the lung and used as starting material for the probing of oligonucleotide microarrays to determine changes in gene expression following phosgene exposure. The data were analyzed using principal component analysis (PCA) to determine the greatest sources of data variability. A three-way analysis of variance (ANOVA) based on exposure, time, and sample was performed to identify the genes most significantly changed as a result of phosgene exposure. These genes were rank ordered by p-values and categorized based on molecular function and biological process. Some of the most significant changes in gene expression reflect changes in glutathione synthesis and redox regulation of the cell, including upregulation of glutathione S-transferase alpha-2, glutathione peroxidase 2, and glutamate-cysteine ligase, catalytic subunit (also known as ó-glutamyl cysteine synthetase). This is in agreement with previous observations describing changes in redox enzyme activity after phosgene exposure. We are also investigating other pathways that are responsive to phosgene exposure to identify mechanisms of toxicity and potential therapeutic targets.,Male CD-1 mice were exposed to air or 32 mg per cubic meter phosgene for 20 min and tissue collected at 30 min, 1 hr, 4 hr, 8 hr, 12 hr, 24 hr, 48 hr, or 72 hr before collection and analysis of lung tissue using oligonucleotide microarrays.,Keyword=phosgene,Keyword=mouse,Keyword=lung,Keyword=carbonyl chloride
2007-06-25 | E-GEOD-2565 | biostudies-arrayexpress
Project description:Time-Dependent Gene Profiling Indicates the Presence of Different Phases for Ischemia/Reperfusion Injury in Retina
2015-07-10 | E-GEOD-43671 | ExpressionAtlas
Project description:Carbonyl chloride (phosgene) is a toxic industrial compound (TIC) widely used in industry for the production of synthetic products, such as polyfoam rubber, plastics, and dyes. Exposure to phosgene results in a latent (1-24 hr), potentially life-threatening pulmonary edema and irreversible acute lung injury. A genomic approach was utilized to investigate the molecular mechanism of phosgene-induced lung injury. CD-1 male mice were exposed whole-body to either air or a concentration x time (c x t) amount of 32 mg/m3 (8 ppm) phosgene for 20 min (640 mg x min/m3). Lung tissue was collected from air- or phosgene-exposed mice at 0.5, 1, 4, 8, 12, 24, 48, and 72 hr post-exposure. RNA was extracted from the lung and used as starting material for the probing of oligonucleotide microarrays to determine changes in gene expression following phosgene exposure. The data were analyzed using principal component analysis (PCA) to determine the greatest sources of data variability. A three-way analysis of variance (ANOVA) based on exposure, time, and sample was performed to identify the genes most significantly changed as a result of phosgene exposure. These genes were rank ordered by p-values and categorized based on molecular function and biological process. Some of the most significant changes in gene expression reflect changes in glutathione synthesis and redox regulation of the cell, including upregulation of glutathione S-transferase alpha-2, glutathione peroxidase 2, and glutamate-cysteine ligase, catalytic subunit (also known as γ-glutamyl cysteine synthetase). This is in agreement with previous observations describing changes in redox enzyme activity after phosgene exposure. We are also investigating other pathways that are responsive to phosgene exposure to identify mechanisms of toxicity and potential therapeutic targets. Male CD-1 mice were exposed to air or 32 mg per cubic meter phosgene for 20 min and tissue collected at 30 min, 1 hr, 4 hr, 8 hr, 12 hr, 24 hr, 48 hr, or 72 hr before collection and analysis of lung tissue using oligonucleotide microarrays. Keyword=phosgene Keyword=mouse Keyword=lung Keyword=carbonyl chloride Keywords: ordered
2005-08-31 | GSE2565 | GEO
Project description:Transcription profiling of human colon Caco2 cells treated with extracts from broccoli that had been cooked for different lengths of time
2025-01-22 | E-MEXP-1372 | ExpressionAtlas
Project description:Microarray profiling of post-injury and uninjured mouse lung neutrophils from a thermal injury model
2020-11-26 | E-MTAB-9770 | ExpressionAtlas
Project description:Transcription profiling by array of human peripheral blood mononuclear cells after treatment with community-associated Staphylococcus aureus and incubation for different lengths of time
2025-01-24 | E-GEOD-16837 | ExpressionAtlas
Project description:Transcription profiling of mouse embyronic stem cells cultured with PI3-K signalling inhibitor LY294002 for different lengths of time to identify PI3K-target genes
2015-11-02 | E-TABM-674 | ExpressionAtlas
Project description:Proteomic analysis of the spatiotemporal based molecular kinetics of acute spinal cord injury identifies a time- and segment-specific window for effective tissue repair
2022-01-14 | E-PROT-94 | ExpressionAtlas
Project description:Molecular Changes of Lung Malignancy in HIV Infection
2020-03-31 | E-GEOD-106937 | ExpressionAtlas