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E-MTAB-9770

ABSTRACT: Microarray profiling of post-injury and uninjured mouse lung neutrophils from a thermal injury model

PROVIDER: E-MTAB-9770 | ExpressionAtlas | 2020-11-26

REPOSITORIES: ExpressionAtlas

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Dataset's files

Source:

			Action	DRS
	E-MTAB-9770-analysis-methods.tsv	Tabular
	E-MTAB-9770-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-9770-configuration.xml	Xml
	E-MTAB-9770-percentile-ranks.tsv	Tabular
	E-MTAB-9770.condensed-sdrf.tsv	Tabular

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Project description:Lungs were collected as soon as possible after thermal injury 30-40 mins post injury for neutrophil isolation. Lungs were minced in HBSS with 1% BSA (Sigma) and strained through a 40um nylon filter (BD). Samples were sorted using BD fusion machine. A 100um nozzle was used at a flow rate of 2.RNA was extracted from these neutrophils and microarray was performed.

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