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E-GEOD-30775

ABSTRACT: Gene expression change after LSD1 siRNA treatment in ER-negative breast cancer cells MDA-MB-231

OTHER RELATED OMICS DATASETS IN: PRJNA144327

PROVIDER: E-GEOD-30775 | ExpressionAtlas | 2025-01-24

REPOSITORIES: ExpressionAtlas

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Dataset's files

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			Action	DRS
	E-GEOD-30775-analysis-methods.tsv	Tabular
	E-GEOD-30775-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-30775-configuration.xml	Xml
	E-GEOD-30775-percentile-ranks.tsv	Tabular
	E-GEOD-30775.condensed-sdrf.tsv	Tabular

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Gene expression change after LSD1 siRNA treatment in ER-negative breast cancer cells MDA-MB-231

Project description:Knock-down of LSD1 using siRNA approach induced regulation of several proliferation-associated genes in ER-negative breast cancer cells MDA-MB-231. To identify changes on gene expression caused by treatment with siRNA directed against LSD1 (si) or control siRNA (control) in MDA-MB-231 cells, total RNA was purified from the cells after treatment for 6 days (2 rounds of transfection). Three biological replicates were used.

2011-07-19 | E-GEOD-30775 | biostudies-arrayexpress

Gene expression change after LSD1 siRNA treatment in ER-negative breast cancer cells MDA-MB-231

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Project description:Gene expression change after LSD1 siRNA treatment in ER-negative breast cancer cells MDA-MB-231

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Project description:MDA-MB-231 are a metastatic triple-negative breast cancer cell line that bears a missense p53 mutation (R280K). We depleted endogenous mutp53 in MDA-MB-231 cells by siRNA transfection, and treated the cells with Tumor Necrosis Factor (TNF)-alpha. Microarray analysis was performed to evaluate the impact of mutant p53 on the transcriptional response triggered by TNFα in these cells. MDA-MB-231 cells transfected with control or p53 siRNA were treated with TNFα for 20 hrs or left untreated. The study comprises four experimental points, each in triplicate.

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