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E-GEOD-33441

ABSTRACT: Expression data from cynosure (cyn) mutant and its CYNOSURE (CYN) wild-type at before flowering and 5 days after pollination stage

PROVIDER: E-GEOD-33441 | ExpressionAtlas | 2015-12-01

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-33441-analysis-methods.tsv	Tabular
	E-GEOD-33441-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-33441-configuration.xml	Xml
	E-GEOD-33441-percentile-ranks.tsv	Tabular
	E-GEOD-33441.condensed-sdrf.tsv	Tabular

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Expression data from cynosure (cyn) mutant and its CYNOSURE (CYN) wild-type at before flowering and 5 days after pollination stage

Project description:To assess further the role of CYN in relation to integument morphogenesis, gametophytic cell fate specification, embryogenesis and endosperm development, we compared the expression profiles between the cyn mutant and wild-type at two different stages(before flowering and 5 days after pollination stage) of ovule development by microarray analysis. Specifically, we confirmed significant changes of up- or down-regulation in some genes required for several biological processes, such as auxin efflux & polarity & cell differentiation, flower and embryonic development, signal transduction.These results supported that MADS protein CYN could function both as a transcriptional activator and repressor, directly and/or indirectly interacted with dozens of potential target genes (including MADS or non-MADS transcription factors) involved in developmental and hormonal pathways. Three independent biological replicates of cyn mutant and wild-type panicle mRNA at two different stages of ovule development were used for microarray experiments. To perform microarray analysis, the samples were pooled by 20 panicles from 10 individual plants at before flowering and 5 days after pollination stage.

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Expression data from cynosure (cyn) mutant and its CYNOSURE (CYN) wild-type at before flowering and 5 days after pollination stage

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