Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from cynosure (cyn) mutant and its CYNOSURE (CYN) wild-type at before flowering and 5 days after pollination stage


ABSTRACT: To assess further the role of CYN in relation to integument morphogenesis, gametophytic cell fate specification, embryogenesis and endosperm development, we compared the expression profiles between the cyn mutant and wild-type at two different stages(before flowering and 5 days after pollination stage) of ovule development by microarray analysis. Specifically, we confirmed significant changes of up- or down-regulation in some genes required for several biological processes, such as auxin efflux & polarity & cell differentiation, flower and embryonic development, signal transduction.These results supported that MADS protein CYN could function both as a transcriptional activator and repressor, directly and/or indirectly interacted with dozens of potential target genes (including MADS or non-MADS transcription factors) involved in developmental and hormonal pathways. Three independent biological replicates of cyn mutant and wild-type panicle mRNA at two different stages of ovule development were used for microarray experiments. To perform microarray analysis, the samples were pooled by 20 panicles from 10 individual plants at before flowering and 5 days after pollination stage.

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: LI JUAN 

PROVIDER: E-GEOD-33441 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The Bsister MADS gene FST determines ovule patterning and development of the zygotic embryo and endosperm.

Lee Dong Sun DS   Chen Li Juan LJ   Li Cheng Yun CY   Liu Yongsheng Y   Tan Xue Lin XL   Lu Bao-Rong BR   Li Juan J   Gan Shu Xian SX   Kang Sang Gu SG   Suh Hak Soo HS   Zhu Youyong Y  

PloS one 20130319 3


Many homeotic MADS-box genes have been identified as controllers of the floral transition and floral development. However, information regarding Bsister (Bs)-function genes in monocots is still limited. Here, we describe the functional characterization of a Bs-group MADS-box gene FEMALE-STERILE (FST), whose frame-shift mutation (fst) results in abnormal ovules and the complete abortion of zygotic embryos and endosperms in rice. Anatomical analysis showed that the defective development in the fst  ...[more]

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