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E-GEOD-39989

ABSTRACT: Transcription profiling by array of Olfm4-deficient murine prostate.

PROVIDER: E-GEOD-39989 | ExpressionAtlas | 2016-11-08

REPOSITORIES: ExpressionAtlas

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Dataset's files

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			Action	DRS
	E-GEOD-39989-analysis-methods.tsv	Tabular
	E-GEOD-39989-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-39989-configuration.xml	Xml
	E-GEOD-39989-percentile-ranks.tsv	Tabular
	E-GEOD-39989.condensed-sdrf.tsv	Tabular

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Project description:Purpose: To explore the mechanisms underlying increased tumorigenesis of Olfm4-deficient murine prostate. Experiment design: Comparison of gene expression between Olfm4-knockout and wild-type mice at 3 months of age in the prostate tissue. Experimental procedures: Total RNA was purified from whole prostate tissue of Olfm4 (+/+) and Olfm4 (-/-) mice at 3 months of age using the RNeasy Plus Mini Kit (Qiagen). Microarray analyses were performed by the NIDDK Core Facility at the National Institutes of Health using Affymetrix Mouse Genome 430 2.0 Array GeneChips (Affymetrix; Santa Clara, CA). Biological replications were used for the Olfm4 (+/+) or Olfm4 (-/-) prostate RNA extracted from five individual mice. The microarray signals were analyzed using the Affymetrix RMA algorithm. The analysis of variance results, False Discovery Rate (FDR) reports, and heatmaps were generated using Partek Genomic of tware 6.5 (Partek; St. Charles, MO). Pathway analyses were performed using MetaCore webaccess software (http://www.genego.com/). Comparison of KO vs. WT with four replications per treatment sample

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