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E-GEOD-55966

ABSTRACT: Gene expression of early craniofacial development through RNA-seq

OTHER RELATED OMICS DATASETS IN: PRJNA241444PRJNA241447PRJNA241443PRJNA241446

PROVIDER: E-GEOD-55966 | ExpressionAtlas | 2016-05-13

REPOSITORIES: ExpressionAtlas

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Dataset's files

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			Action	DRS
	E-GEOD-55966-analysis-methods.tsv	Tabular
	E-GEOD-55966-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-55966-coexpressions.tsv.gz	Tabular
	E-GEOD-55966-configuration.xml	Xml
	E-GEOD-55966-factors.xml	Xml

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Project description:Proper retinoic acid (RA) signaling is essential for normal craniofacial development. Both excessive RA and RA deficiency in early embryonic stage led to a variety of craniofacial malformations, e.g., cleft palate, which have been investigated extensively. Dysregulated Wnt and Shh signaling were shown to underlie the pathogenesis of RA-induced craniofacial defects. In our present study, we showed a spatiotemporal-specific effect of RA signaling in regulating early development of facial prominences. While inhibited the Wnt activities in E12.5/E13.5 mouse palatal shelves, early exposure of excessive RA induced Wnt signaling and Wnt-related gene expression in mouse embryonic Frontonasal/Maxillary processes. A conserved regulatory network of miR-484-Fzd5 was identified to play critical roles in RA regulated craniofacial development using RNA-seq.

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Gene expression of early craniofacial development through RNA-seq

Project description:We report gene expression patterns in embryonic mice (E8.5, E9.5, E10.5) through RNA-seq of 13 tissue/stage combinations in order to generage an atlas of early mouse craniofacial gene expression. Embryos at E8.5, E9.5, and E10.5 CD1 were collected from CD1 mice, fom multiple micro-regions whose development, differentiation and signaling are responsible for the construction of the mammalian face.

2014-03-18 | E-GEOD-55966 | biostudies-arrayexpress

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