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E-GEOD-59089

ABSTRACT: RNA-seq transcriptional profiling in primary human erythroid progenitor cells upon shRNA-mediated knockdown of PRC2 core subunits

OTHER RELATED OMICS DATASETS IN: PRJNA254355PRJNA254352PRJNA254354

PROVIDER: E-GEOD-59089 | ExpressionAtlas | 2019-05-14

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-59089-analysis-methods.tsv	Tabular
	E-GEOD-59089-analytics.tsv	Tabular
	E-GEOD-59089-analytics.tsv.undecorated	Tabular
	E-GEOD-59089-analytics.tsv.undecorated.unrounded	Tabular
	E-GEOD-59089-analytics.tsv.unrounded	Tabular

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Project description:RNA-seq transcriptional profiling in primary human erythroid progenitor cells upon shRNA-mediated knockdown of PRC2 core subunits

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RNA-seq transcriptional profiling in primary human erythroid progenitor cells upon shRNA-mediated knockdown of PRC2 core subunits

Project description:Polycomb Repressive Complex 2 (PRC2) plays crucial roles in transcriptional regulation and stem cell development. However, the context-specific functions associated with alternative subunits remain largely unexplored. Here we show that the related enzymatic subunits EZH1 and EZH2 undergo an expression switch during hematopoiesis. We examine the in vivo stoichiometry of the PRC2 complexes by quantitative proteomics and reveal the existence of an EZH1-SUZ12 sub-complex lacking EED. We provide evidence that EZH1 together with SUZ12 form a non-canonical PRC2 complex, occupy active chromatin domains in the absence of H3K27me3, and positively regulate gene expression. Loss of EZH2 expression leads to global repositioning of EZH1 chromatin occupancy to EZH2 targets. Moreover, we demonstrate that an erythroid-specific enhancer mediates transcriptional activation of EZH1, and a switch from GATA2 to GATA1 controls the developmental EZH1/2 switch by differential association with EZH1 enhancers during erythropoiesis. Thus, the lineage- and developmental stage-specific regulation of PRC2 expression and subunit composition leads to a switch from canonical silencing to non-canonical PRC2 functions during blood stem cell specification. Transcriptional profiling in primary human fetal liver proerythroblasts upon lentiviral shRNA-mediated knockdown of EZH1, EZH2, EED, or SUZ12 by RNA-seq analysis.

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Project description:Primary human erythroid progenitor cells HDAC1 and HDAC2 shRNA knockdown samples

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Project description:This is a dynamic pathway model examining the roles of of the two transcriptional negative feedback regulators of the suppressor of cytokine signaling (SOCS) family, CIS and SOCS3, in JAK/STAT5 signaling, within the context of primary erythroid progenitor cells.

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Project description:ChIP-seq analysis of PRC2 core subunits in primary human erythroid progenitor cells

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