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E-GEOD-63452

ABSTRACT: Knock-in of PIK3CA-H1047R into MCF-10A

OTHER RELATED OMICS DATASETS IN: PRJNA267827

PROVIDER: E-GEOD-63452 | ExpressionAtlas | 2019-05-14

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-63452-analysis-methods.tsv	Tabular
	E-GEOD-63452-analytics.tsv	Tabular
	E-GEOD-63452-analytics.tsv.undecorated	Tabular
	E-GEOD-63452-analytics.tsv.undecorated.unrounded	Tabular
	E-GEOD-63452-analytics.tsv.unrounded	Tabular

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Project description:By survival analysis of breast cancer patients, JMJD6 was found to be significantly associated with poor prognosis. Over-expression and knock-down of JMJD6 in breast cancer cell lines suggested a role in proliferation. In order to study the transcriptional events that occur following JMJD6 expression changes, siRNA-mediated knock-down of JMJD6 was performed in MCF-7 and MDA-MB231 and stable over-expression of JMJD6 was performed in MCF-7. There are 2 different siRNA-mediated knock-downs of JMJD6 with 2 biological replicates in MCF-7 and MDA-MB231; 3 clones of JMJD6 over-expression with 3 biological replicates in MCF-7. The control for the knock-downs is scrambled siRNA-treated MCF-7 and MDA-MB231 and the control for JMJD6 over-expression is empty vector over-expression in MCF-7.

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