Unknown,Transcriptomics,Genomics,Proteomics

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Knock-down and Over-expression of JMJD6 in MCF-7 and/or MDA-MB231

ABSTRACT: By survival analysis of breast cancer patients, JMJD6 was found to be significantly associated with poor prognosis. Over-expression and knock-down of JMJD6 in breast cancer cell lines suggested a role in proliferation. In order to study the transcriptional events that occur following JMJD6 expression changes, siRNA-mediated knock-down of JMJD6 was performed in MCF-7 and MDA-MB231 and stable over-expression of JMJD6 was performed in MCF-7. There are 2 different siRNA-mediated knock-downs of JMJD6 with 2 biological replicates in MCF-7 and MDA-MB231; 3 clones of JMJD6 over-expression with 3 biological replicates in MCF-7. The control for the knock-downs is scrambled siRNA-treated MCF-7 and MDA-MB231 and the control for JMJD6 over-expression is empty vector over-expression in MCF-7.

ORGANISM(S): Homo sapiens

SUBMITTER: Yi Fang Lee 

PROVIDER: E-GEOD-31782 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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JMJD6 is a driver of cellular proliferation and motility and a marker of poor prognosis in breast cancer.

Lee Yi Fang YF   Miller Lance David LD   Chan Xiu Bin XB   Black Michael A MA   Pang Brendan B   Ong Chee Wee CW   Salto-Tellez Manuel M   Liu Edison T ET   Desai Kartiki V KV  

Breast cancer research : BCR 20120523 3

<h4>Introduction</h4>We developed an analytic strategy that correlates gene expression and clinical outcomes as a means to identify novel candidate oncogenes operative in breast cancer. This analysis, followed by functional characterization, resulted in the identification of Jumonji Domain Containing 6 (JMJD6) protein as a novel driver of oncogenic properties in breast cancer.<h4>Methods</h4>Through microarray informatics, Cox proportional hazards regression was used to analyze the correlation b  ...[more]

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