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E-GEOD-7762

ABSTRACT: Transcription profiling of mouse striatum treated with morphine, animals from four inbred strains

OTHER RELATED OMICS DATASETS IN: PRJNA99893

PROVIDER: E-GEOD-7762 | ExpressionAtlas | 2014-08-16

REPOSITORIES: ExpressionAtlas

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Dataset's files

Source:

			Action	DRS
	E-GEOD-7762-analysis-methods.tsv	Tabular
	E-GEOD-7762-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-7762-configuration.xml	Xml
	E-GEOD-7762-configuration.xml.beforeQC	Xml
	E-GEOD-7762-percentile-ranks.tsv	Tabular

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Transcription profiling of mouse striatum treated with morphine, animals from four inbred strains

Project description:Chronic opiate use produces molecular and cellular adaptations in the nervous system, leading to tolerance, physical dependence and addiction. Genome-wide comparison of morphine-induced changes in brain transcription of mouse strains with different opioid-related phenotypes provides an opportunity to discover the relationship between gene expression and behavioral response to the drug. Experiment Overall Design: Microarray experiment was designed to determine the impact of genetic background on the transcriptional effects of morphine in the striatum. The effects of single (20 mg/kg, s.c.) and repeated morphine administration (10-40 mg/kg, 3 times daily for 5 days) were analyzed in four inbred mouse strains (129P3/J. DBA/2J, C57BL/6J, SWR/J). Twelve experimental groups were compared. Control animals received injections of saline. Three independent biological replicates of the microarray were prepared for each experimental group. For each array, independent pools of total RNA from three animals were prepared.

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