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E-MEXP-1512

ABSTRACT: Transcription profiling of human bronchial epithelial cells following exposure to whole cigarette smoke

PROVIDER: E-MEXP-1512 | ExpressionAtlas | 2025-01-22

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MEXP-1512-analysis-methods.tsv	Tabular
	E-MEXP-1512-atlasExperimentSummary.Rdata	Rdata
	E-MEXP-1512-configuration.xml	Xml
	E-MEXP-1512-percentile-ranks.tsv	Tabular
	E-MEXP-1512-zoomifications-log.tsv	Tabular

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Organotypic human bronchial epithelial cultures (MucilAir) exposed to mainstream aerosols from the 3R4F cigarette and a novel tobacco vapor product

Project description:Cigarette smoke (CS) is an aerosol containing more than 6,000 chemicals and one of the risk factor in the development of chronic inflammatory lung disease. To evaluate biological effect of CS on human respiratory tract, organotypic bronchial epithelial cultures can be used to replicate in vivo tissue conditions. The MucilAir organotypic bronchial epithelial cultures were exposed to mainstream aerosols from the 3R4F cigarette and a novel tobacco vapor product (NTV), which we recently developed, using a Vitrocell exposure system. This system consists of three steps: the generation of CS, dilution, and exposure to an air-liquid interface cultured cells in a specially designed module. This exposure scenario mimics CS exposure in the human airway (i.e. direct aerosol exposure to the apical surface of air-liquid interface-cultured cells), We found a dose-dependent increase in the number of differentially expressed genes following 3R4F cigarette smoke exposure, compared with expression in air-exposed controls. In contrast, no changes were detected following exposure to NTV vapor.

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