Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-MTAB-2790

ABSTRACT: Transcription profiling by array of mouse embryonic fibroblasts from p27 -/- knockouts and wild-type controls to study the role of p27 in regulating the cell cycle

PROVIDER: E-MTAB-2790 | ExpressionAtlas | 2014-07-25

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E-MTAB-2790-analysis-methods.tsv	Tabular
	E-MTAB-2790-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-2790-configuration.xml	Xml
	E-MTAB-2790-percentile-ranks.tsv	Tabular
	E-MTAB-2790.condensed-sdrf.tsv	Tabular

Items per page:

1 - 5 of 56

Similar Datasets

p27Kip1 regulates cell cycle progression by modulating the expression of p21Cip1

Project description:In addition to its classical role as a CDK inhibitor, p27 also acts as a transcriptional regulator. We found that at early passages, embryonic fibroblasts from p27-/- mouse show reduced expression of genes involved in DNA replication and progress slowly through the cell cycle. These cells have high levels of p21 that associate with cyclin-cdk2 complexes and reduce their activity. Decreasing p21 in these cells elevates cdk2 activity and DNA replication. We observed that silencing p27 in cells induces p21 transcription. Interestingly, the transcription factor Pitx2 is up-regulated in p27-/- cells. We found that p27 associates with a regulatory domain of the Pitx2 gene and represses its expression. In turn, Pitx2 associates with p21 promoter and induces its transcription. Moreover, reducing Pitx2 in p27-/- cells decreases p21 levels. These findings indicate that p27 controls cell cycle progression by regulating Pitx2-mediated expression of p21.

2016-06-09 | E-MTAB-2790 | biostudies-arrayexpress

E-GEOD-43883

Project description:Transcription profiling by array of three Arabidopsis knockouts against wild type controls to study the influence of ubiquitin-like proteases in plant transcriptional modulation

2015-04-07 | E-GEOD-43883 | ExpressionAtlas

E-MTAB-6259

Project description:Molecular profiling of Ctcf hemizygous mouse embryonic fibroblasts: RNA-seq

2018-09-17 | E-MTAB-6259 | ExpressionAtlas

E-GEOD-63756

Project description:Transcription profiling by high throughput sequencing of mouse embryonic fibroblasts treated with tunicamycin to study the transcriptional response to endoplasmic reticulum stress

2019-05-14 | E-GEOD-63756 | ExpressionAtlas

E-GEOD-15325

Project description:Transcription profiling by array of wild type, K-ras mutant, Wt1-null, and double K-ras mutant/Wt1-null mouse embryonic fibroblasts

2014-07-03 | E-GEOD-15325 | ExpressionAtlas

E-MEXP-793

Project description:Transcription profiling by array of mouse embryonic fibroblasts expressing CBX8 or BMI1

2014-07-03 | E-MEXP-793 | ExpressionAtlas

E-GEOD-27843

Project description:Transcription profiling by high throughput sequencing of mouse embryonic fibroblasts, terminally differentiated neurons and embryonic stem cells

2016-05-13 | E-GEOD-27843 | ExpressionAtlas

E-MTAB-5210

Project description:Transcription profiling of wild type and Stella knockout oocytes, wild type and Stella maternal/zygotic knockout embryos to study to the role of Stella in early mouse development

2019-05-14 | E-MTAB-5210 | ExpressionAtlas

E-GEOD-54628

Project description:Expression data from E16.5 mouse embryonic brain wild-type and knock-out conditions

2015-07-31 | E-GEOD-54628 | ExpressionAtlas

E-GEOD-8972

Project description:Transcription profiling of mouse developing and mature Nrl -/- retina against wild type controls

2014-08-16 | E-GEOD-8972 | ExpressionAtlas