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E-MTAB-3103

ABSTRACT: RNA-seq of inner pericarp, outer pericarp and endosperm layers from developing grain of bread wheat cv. Holdfast at 12 days post-anthesis

PROVIDER: E-MTAB-3103 | ExpressionAtlas | 2019-01-08

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-3103-analysis-methods.tsv	Tabular
	E-MTAB-3103-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-3103-coexpressions.tsv.gz	Tabular
	E-MTAB-3103-configuration.xml	Xml
	E-MTAB-3103-factors.xml	Xml

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Tissue layers from developing wheat grain at 12 days post-anthesis

Project description:Inner pericarp, outer pericarp and endosperm layers from developing grain of bread wheat cv. Holdfast at 12 days post-anthesis.

2015-04-24 | E-MTAB-3103 | biostudies-arrayexpress

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Project description:Grain filling and proper grain development are essential biological processes in the plant’s life cycle, which majorly contributes to the final seed yield and quality in all cereal crop. However, very scarcely this knowledge is available in the literature regarding how the different wheat grain components contribute to the overall development of the seed. We performed a proteomics and metabolomics analysis in four different developing components of the wheat grain (seed coat, embryo, endosperm and cavity fluid) to characterize molecular processes during early and late grain development. In-gel shotgun proteomics analysis in 12, 15, 20 and 25 days after anthesis (DAA) lead us to identify and quantify 15,484 proteins out of which 410 differentially expressed proteins (DEPs) were identified in the seed coat, 815 in embryo, 372 in endosperm and 492 in cavity fluid. The abundance of selected protein candidates revealed spatially and temporally resolved protein functions associated with development and grain filling. Multiple proteins such as pyruvate phosphate dikinase (PPDK) and 14 -3- 3 undergo a major change in abundance during wheat grain development. Proteins binned into the functional category of cell growth /division were highly expressed during early stages (12 and 15 DAA) whereas those of starch biosynthesis in the middle or late stages. At the metabolome level all tissues and especially the cavity fluid showed highly distinct metabolite profiles. The tissue specific data are integrated with biochemical networks to explore a comprehensive map of molecular processes during grain filling and developmental processes.

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Tissue layers from developing wheat grain at 12 days post-anthesis

Project description:Tissue layers from developing wheat grain at 12 days post-anthesis

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Project description:The high molecular weight (HMW) subunits of wheat glutenin are synthesised only in the starchy endosperm tissue of the developing wheat grain. We compared the expressed genomes of the transgenic wheat line B102,1-1 (Rooke et al. Transgene inheritance, segregation and expression in bread wheat. Euphytica 129, 301-309 (2003)). Both lines were shown to express the HMW-GS Ax1 gene (Halford, N.G. et al. Analysis of HMW glutenin subunits encoded bychromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality. Theor Appl Genet 83, 373-378 (1992).) to the expressed genome of conventionally bred wheat line L88-18 (Lawrence et al. Dough and baking quality of wheat lines in glutenin subunits controlled by Glu-A1, Glu-B1 and Glu-D1 loci. J. Cereal. Sci. 7,109-112 (1988)) which results in the same effects on traits. Transcriptomes comparison analysis was performed in endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination dpg), respectively. Each of the transcriptome comparisons was performed using three biological replicates (i.e. per line/tissue /developmental stage selected). Hybridisations were performed in reverse dye labelling.Exceptionally, biological replica 2 was only performed for B102,1-1 (green)/L88-18 (red) labelling and not swap

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