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Wheat grain cell wall enriched proteome

ABSTRACT: The aim of this project is to highlight cell wall proteome of wheat developing at two key stage of its development (150 GDD : middle of endosperm cellularisation and 250 GDD : start of grain filling with storage compounds). It's the first study of wheat grain cell wall proteome in which endosperm were separated of outer layers in order to gain more information about the mechanisms of cell wall assembly and remodeling.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Triticum Aestivum (wheat)

TISSUE(S): Plant Cell

SUBMITTER: Mehdi CHERKAOUI

LAB HEAD: Fabienne Guillon

PROVIDER: PXD016258 | Pride | 2020-05-27

REPOSITORIES: Pride

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	OB09565.mzXML	Mzxml
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Publications

Cell Wall Proteome of Wheat Grain Endosperm and Outer Layers at Two Key Stages of Early Development.

Cherkaoui Mehdi M Lollier Virginie V Geairon Audrey A Bouder Axelle A Larré Colette C Rogniaux Hélène H Jamet Elisabeth E Guillon Fabienne F Francin-Allami Mathilde M

International journal of molecular sciences 20191229 1

The cell wall is an important compartment in grain cells that fulfills both structural and functional roles. It has a dynamic structure that is constantly modified during development and in response to biotic and abiotic stresses. Non-structural cell wall proteins (CWPs) are key players in the remodeling of the cell wall during events that punctuate the plant life. Here, a subcellular and quantitative proteomic approach was carried out to identify CWPs possibly involved in changes in cell wall m ...[more]

PMID: 31905787

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Cell wall proteome of wheat developing grain

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Project description:The high molecular weight (HMW) subunits of wheat glutenin are synthesised only in the starchy endosperm tissue of the developing wheat grain. We compared the expressed genomes of the transgenic wheat line B102,1-1 (Rooke et al. Transgene inheritance, segregation and expression in bread wheat. Euphytica 129, 301-309 (2003)). Both lines were shown to express the HMW-GS Ax1 gene (Halford, N.G. et al. Analysis of HMW glutenin subunits encoded bychromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality. Theor Appl Genet 83, 373-378 (1992).) to the expressed genome of conventionally bred wheat line L88-18 (Lawrence et al. Dough and baking quality of wheat lines in glutenin subunits controlled by Glu-A1, Glu-B1 and Glu-D1 loci. J. Cereal. Sci. 7,109-112 (1988)) which results in the same effects on traits. Transcriptomes comparison analysis was performed in endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination dpg), respectively. Each of the transcriptome comparisons was performed using three biological replicates (i.e. per line/tissue /developmental stage selected). Hybridisations were performed in reverse dye labelling.Exceptionally, biological replica 2 was only performed for B102,1-1 (green)/L88-18 (red) labelling and not swap

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