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E-MTAB-5755

ABSTRACT: Microarrays of mouse embryonic limb skeletal muscle at days E14.5 and E18.5 lacking the RYR1 or Cav1.1 Ca2+ channels

PROVIDER: E-MTAB-5755 | ExpressionAtlas | 2017-11-08

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-5755-analysis-methods.tsv	Tabular
	E-MTAB-5755-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-5755-configuration.xml	Xml
	E-MTAB-5755-percentile-ranks.tsv	Tabular
	E-MTAB-5755.condensed-sdrf.tsv	Tabular

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Microarrays of mouse embryonic limb skeletal muscle at days E14.5 and E18.5 lacking the RYR1 or Cav1.1 Ca2+ channels

Project description:Our aim is to analyze how does the lack of each of the Ca2+ channels, involved in excitation-contraction coupling in skeletal muscle - RYR1 and Cav1.1, affect gene expression in embryonic mouse limb skeletal muscle during secondary myogenesis. We extracted total RNA from the limb skeletal muscle of WT, heterozygous RYR1-/+ and Cav1.1+/-, and homozygous RYR1-/- and Cav1.1-/- mutants from 3 litters (n = 3 for each group) at days E14.5 and E18.5 and subjected it to microarray analyses.

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